@article{a33ab32ace754b93a4a91a457d41d720,
title = "Cerebrospinal fluid regulates skull bone marrow niches via direct access through dural channels",
abstract = "It remains unclear how immune cells from skull bone marrow niches are recruited to the meninges. Here we report that cerebrospinal fluid (CSF) accesses skull bone marrow via dura–skull channels, and CSF proteins signal onto diverse cell types within the niches. After spinal cord injury, CSF-borne cues promote myelopoiesis and egress of myeloid cells into meninges. This reveals a mechanism of CNS-to-bone-marrow communication via CSF that regulates CNS immune responses.",
author = "Mazzitelli, {Jose A.} and Smyth, {Leon C.D.} and Cross, {Kevin A.} and Taitea Dykstra and Jerry Sun and Siling Du and Tornike Mamuladze and Igor Smirnov and Justin Rustenhoven and Jonathan Kipnis",
note = "Funding Information: We thank all the members of the Kipnis Laboratory for their valuable comments during many discussions of this work. We thank P. Bayguinov for assistance with two-photon microscopy. We thank R. R. Townsend, Q. Zhang and P. Erdmann-Gilmore for assistance with LC–MS experiments and analysis. We also thank McDonnell Genome Institute for processing scRNA-seq and the Flow Cytometry Core of the Department of Pathology and Immunology, School of Medicine, Washington University in St. Louis, for assistance with cell sorting. We also acknowledge D. Bender and the Bursky Center for Human Immunology & Immunotherapy Programs for assistance with the Luminex analysis. This work was funded by National Institutes of Health grants AT010416 and NS096967, a Cure Alzheimer{\textquoteright}s Fund (Berg Brain Entry and Exit Consortium) grant and the BJC HealthCare Investigators Program, all to J.K, as well as by National Institutes of Health grant T32NS121881, to J.A.M. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Funding Information: We thank all the members of the Kipnis Laboratory for their valuable comments during many discussions of this work. We thank P. Bayguinov for assistance with two-photon microscopy. We thank R. R. Townsend, Q. Zhang and P. Erdmann-Gilmore for assistance with LC?MS experiments and analysis. We also thank McDonnell Genome Institute for processing scRNA-seq and the Flow Cytometry Core of the Department of Pathology and Immunology, School of Medicine, Washington University in St. Louis, for assistance with cell sorting. We also acknowledge D. Bender and the Bursky Center for Human Immunology & Immunotherapy Programs for assistance with the Luminex analysis. This work was funded by National Institutes of Health grants AT010416 and NS096967, a Cure Alzheimer?s Fund (Berg Brain Entry and Exit Consortium) grant and the BJC HealthCare Investigators Program, all to J.K, as well as by National Institutes of Health grant T32NS121881, to J.A.M. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2022",
month = may,
doi = "10.1038/s41593-022-01029-1",
language = "English",
volume = "25",
pages = "555--560",
journal = "Nature Neuroscience",
issn = "1097-6256",
number = "5",
}