Central metabolism in mycobacterium smegmatis during the transition from O2-rich to O2-poor conditions as studied by isotopomer-assisted metabolite analysis

Yinjie J. Tang, Wenqing Shui, Samuel Myers, Xueyang Feng, Carolyn Bertozzi, Jay D. Keasling

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Isotopomer-assisted metabolite analysis was used to investigate the central metabolism of Mycobacterium smegmatis and its transition from normal growth to a non-replicating state under a hypoxic environment. Tween 80 significantly promoted aerobic growth by improving O2 transfer, while only small amount was degraded and metabolized via the TCA cycle for biomass synthesis. As the bacillus encountered hypoxic stress, isotopomer analysis suggested: (1) isocitrate lyase activity increased, which further induced glyoxylate pathway and glycine dehydrogenase for replenishing NAD+; (2) the relative amount of acetyl-CoA entering the TCA cycle was doubled, whereas little entered the glycolytic and pentose phosphate pathways.

Original languageEnglish
Pages (from-to)1233-1240
Number of pages8
JournalBiotechnology Letters
Volume31
Issue number8
DOIs
StatePublished - Jul 2009

Keywords

  • Glycine
  • Glyoxylate
  • NAD
  • Non-replicating
  • Tween 80

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