Cellular versus myocardial basis for the contractile dysfunction of hypertrophied myocardium

D. L. Mann, Y. Urabe, R. L. Kent, S. Vinciguerra, G. Cooper IV

Research output: Contribution to journalArticle

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Abstract

Contractile dysfunction has been demonstrated in many previous studies of experimental right ventricular pressure-overload hypertrophy; however, given the complex changes that occur both in the cardiac muscle cell and in the multiple components of the cardiac interstitium, it is not clear whether the contractile dysfunction observed is an intrinsic property of the cardiac muscle cell or whether it is the result of a mechanicallly normal cardiac muscle cell contracting within an abnormal interstitial environment. The purpose of the present study was to examine the contractile behavior of cardiac muscle cells, or cardiocytes, isolated from seven cat right ventricles that were pressure-overloaded by banding the pulmonary artery; right ventricular cardiocytes from seven sham-operated cats served as controls. Cardiocytes were obtained from these cats via standard cell isolation procedures; contractile function of the cardiocytes in response to graded viscous external loads was defined by laser diffraction. The cells were stimulated to contract at a frequency of 0.25 Hz, using 100-μA direct current pulses of alternating polarity. Hypertrophied right ventricular cardiocytes obtained from banded cats showed marked systolic contractile abnormalities in comparison with right ventricular cardiocytes from sham-operated cats. The peak velocity of sarcomere shortening for the control and hypertrophied cardiocytes in 1-cp superfusate was 3.6±0.2 and 2.1±0.1 μm/sec, respectively (p<0.001); the maximum extent of sarcomere shortening for the control and hypertrophied cardiocytes was 0.21±0.01 and 0.14±0.01 μm, respectively (p<0.001). Further, the time to peak shortening in the 1-cp superfusate was significantly longer for the hypertrophied cardiocytes (150.1±3.3 versus 160.4±3.7 msec; p<0.04). When the relengthening properties of the cells were examined in the 1-cp superfusate, there were significant differences between cardiocyte groups. The peak rate of sarcomere relengthening was 3.5±0.2 μm/sec in the control cardiocytes and 2.2±0.17 μm/sec in the hypertrophied cardiocytes (p<0.001). Similarly, the time to peak velocity of sarcomere relengthening (48.8±1.8 versus 57.9±2.9 msec) and the time to 50% maximal sarcomere relengthening (57.1±3.1 versus 67.1±3.1 msec) were both significantly prolonged for the hypertrophied cardiocytes (p<0.02). This study shows for the first time that the contractile defect in this model of right ventricular pressure-overload hypertrophy is intrinsic to the cardiac muscle cell itself. This finding provides a basis for further, more focused investigations designed to determine the mechanisms responsible for the contractile dysfunction observed in this form of experimental cardiac hypertrophy.

Original languageEnglish
Pages (from-to)402-415
Number of pages14
JournalCirculation research
Volume68
Issue number2
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Keywords

  • cardiocytes
  • contractile dysfunction
  • hypertrophy
  • laser diffraction

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