TY - JOUR
T1 - Cellular Requirements for Building a Retinal Neuropil
AU - Randlett, Owen
AU - MacDonald, Ryan B.
AU - Yoshimatsu, Takeshi
AU - Almeida, Alexandra D.
AU - Suzuki, Sachihiro C.
AU - Wong, Rachel O.
AU - Harris, William A.
N1 - Funding Information:
We thank M. Agathocleous for suggesting the Notch inactivation experiments; C. O’Hare and H. Boije for critical readings of the manuscript; A. McNabb, T. Dyl, K.L. Scott, and S. Waldron for fish maintenance; and J. He, C. Norden, P. Jusuf, and members of the Wong, Harris, and Holt laboratories for helpful advice and discussions. We also thank K. Verhey for the Kif5c-mCherry construct, T. Nicholson for the RibeyeA antibody, J. Saari for the Cralbp antibody, J. Fadool for the 5E11 antibody, S. Higashijima for the vsx1:GFP transgenic, S. Leach for the ptf1a:DsRed transgenic, and the Wellcome Trust Sanger Institute Zebrafish Mutation Project for the ptf1a mutant line. We thank E. Parker for technical assistance with electron microscopy. This work was funded by grants from the Wellcome Trust (to W.A.H.), the NIH (EY14358 to R.O.W. and Vision Core grant EY01730 to the University of Washington), and the Wellcome Trust Programme in Developmental Biology and the Cambridge Overseas Trust (O.R.).
PY - 2013
Y1 - 2013
N2 - How synaptic neuropil is formed within the CNS is poorly understood. The retinal inner plexiform layer (IPL) is positioned between the cell bodies of amacrine cells (ACs) and retinal ganglion cells (RGCs). It consists of bipolar cell (BC) axon terminals that synapse on the dendrites of ACs and RGCs intermingled with projections from Müller glia (MG). We examined whether any of these cellular processes are specifically required for the formation of the IPL. Using genetic and pharmacological strategies, we eliminated RGCs, ACs, and MG individually or in combination. Even in the absence of all of these partner cells, an IPL-like neuropil consisting of only BC axon terminals still forms, complete with presynaptic specializations and sublaminar organization. Previous studies have shown that an IPL can form in the complete absence of BCs; therefore, we conclude that neither presynaptic nor postsynaptic processes are individually essential for the formation of this synaptic neuropil.
AB - How synaptic neuropil is formed within the CNS is poorly understood. The retinal inner plexiform layer (IPL) is positioned between the cell bodies of amacrine cells (ACs) and retinal ganglion cells (RGCs). It consists of bipolar cell (BC) axon terminals that synapse on the dendrites of ACs and RGCs intermingled with projections from Müller glia (MG). We examined whether any of these cellular processes are specifically required for the formation of the IPL. Using genetic and pharmacological strategies, we eliminated RGCs, ACs, and MG individually or in combination. Even in the absence of all of these partner cells, an IPL-like neuropil consisting of only BC axon terminals still forms, complete with presynaptic specializations and sublaminar organization. Previous studies have shown that an IPL can form in the complete absence of BCs; therefore, we conclude that neither presynaptic nor postsynaptic processes are individually essential for the formation of this synaptic neuropil.
UR - http://www.scopus.com/inward/record.url?scp=84874259720&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2013.01.020
DO - 10.1016/j.celrep.2013.01.020
M3 - Article
C2 - 23416047
AN - SCOPUS:84874259720
SN - 2211-1247
VL - 3
SP - 282
EP - 290
JO - Cell Reports
JF - Cell Reports
IS - 2
ER -