Cellular and matrix contributions to tissue construct stiffness increase with cellular concentration

J. Pablo Marquez, Guy M. Genin, Kenneth M. Pryse, Elliot L. Elson

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The mechanics of bio-artificial tissue constructs result from active and passive contributions of cells and extracellular matrix (ECM). We delineated these for a fibroblast-populated matrix (FPM) consisting of chick embryo fibroblast cells in a type I collagen ECM through mechanical testing, mechanical modeling, and selective biochemical elimination of tissue components. From a series of relaxation tests, we found that contributions to overall tissue mechanics from both cells and ECM increase exponentially with the cell concentration. The force responses in these relaxation tests exhibited a logarithmic decay over the 3600 second test duration. The amplitudes of these responses were nearly linear with the amplitude of the applied stretch. The active component of cellular forces rose dramatically for FPMs containing higher cell concentrations.

Original languageEnglish
Pages (from-to)1475-1482
Number of pages8
JournalAnnals of biomedical engineering
Volume34
Issue number9
DOIs
StatePublished - Sep 2006

Keywords

  • Cell mechanics
  • Constitutive modeling
  • Matrix mechanics

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