TY - JOUR
T1 - Cellular and cytokine responses to Salmonella enterica serotype typhi proteins in patients with typhoid fever in Bangladesh
AU - Bhuiyan, Md Saruar
AU - Sayeed, Md Abu
AU - Khanam, Farhana
AU - Leung, Daniel T.
AU - Bhuiyan, Taufiqur Rahman
AU - Sheikh, Alaullah
AU - Salma, Umme
AU - LaRocque, Regina C.
AU - Harris, Jason B.
AU - Pacek, Marcin
AU - Calderwood, Stephen B.
AU - LaBaer, Joshua
AU - Ryan, Edward T.
AU - Qadri, Firdausi
AU - Charles, Richelle C.
PY - 2014/6
Y1 - 2014/6
N2 - We assessed interferon-gamma (IFN-γ) responses via enzyme-linked immunosorbent spot (ELISPOT) to a number of S. Typhi antigens in samples from humans with S. Typhi bacteremia and typhoid fever in Bangladesh. Compared with responses in healthy endemic zone controls, there were significantly increased IFN-γ responses at the time of clinical presentation (acute phase) and at convalescence 14-28 days later. The majority (80-90%) of IFN-γ expressing T cells were CD4+. We observed a significant increase in interleukin-17 (IL-17) positive CD4 + T cells at convalescent versus acute stage of infection using an intracellular cytokine staining assay. We also found that stimulated peripheral blood mononuclear cells (PBMCs) produced significantly increased levels of a number of cytokines at the convalescent versus acute phase of infection, including IFN-γ, MIP-1β, sCD40L, TNF-β, IL-13, and IL-9. These results suggest that S. Typhi antigens induce a predominantly Th1 response, but that elevations in other cytokines may be modulatory.
AB - We assessed interferon-gamma (IFN-γ) responses via enzyme-linked immunosorbent spot (ELISPOT) to a number of S. Typhi antigens in samples from humans with S. Typhi bacteremia and typhoid fever in Bangladesh. Compared with responses in healthy endemic zone controls, there were significantly increased IFN-γ responses at the time of clinical presentation (acute phase) and at convalescence 14-28 days later. The majority (80-90%) of IFN-γ expressing T cells were CD4+. We observed a significant increase in interleukin-17 (IL-17) positive CD4 + T cells at convalescent versus acute stage of infection using an intracellular cytokine staining assay. We also found that stimulated peripheral blood mononuclear cells (PBMCs) produced significantly increased levels of a number of cytokines at the convalescent versus acute phase of infection, including IFN-γ, MIP-1β, sCD40L, TNF-β, IL-13, and IL-9. These results suggest that S. Typhi antigens induce a predominantly Th1 response, but that elevations in other cytokines may be modulatory.
UR - http://www.scopus.com/inward/record.url?scp=84902287072&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.13-0261
DO - 10.4269/ajtmh.13-0261
M3 - Article
C2 - 24615129
AN - SCOPUS:84902287072
SN - 0002-9637
VL - 90
SP - 1024
EP - 1030
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 6
ER -