CellTag Indexing: Genetic barcode-based sample multiplexing for single-cell genomics

Chuner Guo, Wenjun Kong, Kenji Kamimoto, Guillermo C. Rivera-Gonzalez, Xue Yang, Yuhei Kirita, Samantha A. Morris

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

High-throughput single-cell assays increasingly require special consideration in experimental design, sample multiplexing, batch effect removal, and data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, CellTag Indexing, which uses predefined genetic barcodes that are heritable, enabling cell populations to be tagged, pooled, and tracked over time in the same experimental replicate. We demonstrate the utility of CellTag Indexing by sequencing transcriptomes using a variety of cell types, including long-term tracking of cell engraftment and differentiation in vivo. Together, this presents CellTag Indexing as a broadly applicable genetic multiplexing tool that is complementary with existing single-cell technologies.

Original languageEnglish
Article number90
JournalGenome biology
Volume20
Issue number1
DOIs
StatePublished - May 9 2019

Fingerprint

Dive into the research topics of 'CellTag Indexing: Genetic barcode-based sample multiplexing for single-cell genomics'. Together they form a unique fingerprint.

Cite this