Cell-Type-Specific Profiling of Alternative Translation Identifies Regulated Protein Isoform Variation in the Mouse Brain

Darshan Sapkota; Allison M. Lake; Wei Yang; Chengran Yang; Hendrik Wesseling; Amanda Guise; Ceren Uncu; Jasbir S. Dalal; Andrew W. Kraft; Jin Moo Lee; Mark S. Sands; Judith A. Steen; Joseph D. Dougherty

doi:10.1016/j.celrep.2018.12.077

Cell-Type-Specific Profiling of Alternative Translation Identifies Regulated Protein Isoform Variation in the Mouse Brain

Darshan Sapkota, Allison M. Lake, Wei Yang, Chengran Yang, Hendrik Wesseling, Amanda Guise, Ceren Uncu, Jasbir S. Dalal, Andrew W. Kraft, Jin Moo Lee, Mark S. Sands, Judith A. Steen, Joseph D. Dougherty

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

By deep sequencing ribosome-bound mRNA fragments, Sapkota et al. demonstrate non-canonical translation initiation and stop codon readthrough that diversify proteins in the mouse brain. They also show that neuronal stimulation regulates the choice of initiation sites, whereas gliotic diseases differentially regulate the levels of normal and readthrough AQP4 isoforms.

Original language	English
Pages (from-to)	594-607.e7
Journal	Cell Reports
Volume	26
Issue number	3
DOIs	https://doi.org/10.1016/j.celrep.2018.12.077
State	Published - Jan 15 2019

Keywords

Aqp4
brain
cell-type specific
gliosis
initiation
neuronal activity
protein variants
readthrough
translation

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10.1016/j.celrep.2018.12.077

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Sapkota, D., Lake, A. M., Yang, W., Yang, C., Wesseling, H., Guise, A., Uncu, C., Dalal, J. S., Kraft, A. W., Lee, J. M., Sands, M. S., Steen, J. A., & Dougherty, J. D. (2019). Cell-Type-Specific Profiling of Alternative Translation Identifies Regulated Protein Isoform Variation in the Mouse Brain. Cell Reports, 26(3), 594-607.e7. https://doi.org/10.1016/j.celrep.2018.12.077

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abstract = "By deep sequencing ribosome-bound mRNA fragments, Sapkota et al. demonstrate non-canonical translation initiation and stop codon readthrough that diversify proteins in the mouse brain. They also show that neuronal stimulation regulates the choice of initiation sites, whereas gliotic diseases differentially regulate the levels of normal and readthrough AQP4 isoforms.",

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doi = "10.1016/j.celrep.2018.12.077",

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AU - Lake, Allison M.

AU - Yang, Wei

AU - Yang, Chengran

AU - Wesseling, Hendrik

AU - Guise, Amanda

AU - Uncu, Ceren

AU - Dalal, Jasbir S.

AU - Kraft, Andrew W.

AU - Lee, Jin Moo

AU - Sands, Mark S.

AU - Steen, Judith A.

AU - Dougherty, Joseph D.

PY - 2019/1/15

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KW - brain

KW - cell-type specific

KW - gliosis

KW - initiation

KW - neuronal activity

KW - protein variants

KW - readthrough

KW - translation

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ER -

Cell-Type-Specific Profiling of Alternative Translation Identifies Regulated Protein Isoform Variation in the Mouse Brain

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Keywords

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