@article{7cc2d9a080854df08baa831e0923ccc2,
title = "Cell-Type-Specific Profiling of Alternative Translation Identifies Regulated Protein Isoform Variation in the Mouse Brain",
abstract = "By deep sequencing ribosome-bound mRNA fragments, Sapkota et al. demonstrate non-canonical translation initiation and stop codon readthrough that diversify proteins in the mouse brain. They also show that neuronal stimulation regulates the choice of initiation sites, whereas gliotic diseases differentially regulate the levels of normal and readthrough AQP4 isoforms.",
keywords = "Aqp4, brain, cell-type specific, gliosis, initiation, neuronal activity, protein variants, readthrough, translation",
author = "Darshan Sapkota and Lake, {Allison M.} and Wei Yang and Chengran Yang and Hendrik Wesseling and Amanda Guise and Ceren Uncu and Dalal, {Jasbir S.} and Kraft, {Andrew W.} and Lee, {Jin Moo} and Sands, {Mark S.} and Steen, {Judith A.} and Dougherty, {Joseph D.}",
note = "Funding Information: We thank Gary Loughran for the dual-luciferase plasmid, the Hope Center Viral Vectors Core at Washington University in St. Louis for viral vector preparation, Mike Vasek for assistance with image analysis, Yating Liu for TIS feature analysis, and members of the J.D.D. laboratory for scientific discussions. This work was supported by the NIH (1R21DA038458, 1R01NS102272), Hope Center, and Children's Discovery Institute of Washington University in St. Louis (to J.D.D.), McDonnell Center for Cellular and Molecular Neurobiology postdoctoral fellowship (to D.S.), and NIH R01GM112007R (to J.A.S.), R01NS084028 (to J.-M.L.), R01NS043205 (to M.S.S.), and P30CA91842 and UL1TR000448 (to the Genome Technology Resource Center at Washington University in St. Louis). J.D.D. is a NARSAD Independent Investigator of the Brain and Behavior Research Foundation. Funding Information: We thank Gary Loughran for the dual-luciferase plasmid, the Hope Center Viral Vectors Core at Washington University in St. Louis for viral vector preparation, Mike Vasek for assistance with image analysis, Yating Liu for TIS feature analysis, and members of the J.D.D. laboratory for scientific discussions. This work was supported by the NIH ( 1R21DA038458 , 1R01NS102272 ), Hope Center , and Children{\textquoteright}s Discovery Institute of Washington University in St. Louis (to J.D.D.), McDonnell Center for Cellular and Molecular Neurobiology postdoctoral fellowship (to D.S.), and NIH R01GM112007R (to J.A.S.), R01NS084028 (to J.-M.L.), R01NS043205 (to M.S.S.), and P30CA91842 and UL1TR000448 (to the Genome Technology Resource Center at Washington University in St. Louis). J.D.D. is a NARSAD Independent Investigator of the Brain and Behavior Research Foundation. Publisher Copyright: {\textcopyright} 2018 The Author(s)",
year = "2019",
month = jan,
day = "15",
doi = "10.1016/j.celrep.2018.12.077",
language = "English",
volume = "26",
pages = "594--607.e7",
journal = "Cell Reports",
issn = "2211-1247",
number = "3",
}