Cell-Type-Specific Profiling of Alternative Translation Identifies Regulated Protein Isoform Variation in the Mouse Brain

Darshan Sapkota, Allison M. Lake, Wei Yang, Chengran Yang, Hendrik Wesseling, Amanda Guise, Ceren Uncu, Jasbir S. Dalal, Andrew W. Kraft, Jin Moo Lee, Mark S. Sands, Judith A. Steen, Joseph D. Dougherty

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

By deep sequencing ribosome-bound mRNA fragments, Sapkota et al. demonstrate non-canonical translation initiation and stop codon readthrough that diversify proteins in the mouse brain. They also show that neuronal stimulation regulates the choice of initiation sites, whereas gliotic diseases differentially regulate the levels of normal and readthrough AQP4 isoforms.

Original languageEnglish
Pages (from-to)594-607.e7
JournalCell Reports
Volume26
Issue number3
DOIs
StatePublished - Jan 15 2019

Keywords

  • Aqp4
  • brain
  • cell-type specific
  • gliosis
  • initiation
  • neuronal activity
  • protein variants
  • readthrough
  • translation

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