Cell surface proteins binding to recombinant soluble HIV-1 and HIV-2 transmembrane proteins

C. F. Ebenbichler, C. Roder, R. Vornhagen, L. Ratner, M. P. Dierich

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Objective: To further characterize cell surface proteins binding to recombinant soluble (rs) forms of the transmembrane glycoproteins gp41 of HIV-1 (rsgp41) and gp36 of HIV-2 (rsgp36). Methods: Various human and murine cell lines of different lineages were surface-labelled with 125l. rsgp41 and rsgp36 were bound to CnBr-Sepharose and used as an affinity matrix for the surface-labelled cell lysates. The bound cell surface proteins were separated on sodium dodecyl sulphate polyacrylamide gel electrophoresis under reducing conditions. A rabbit serum was produced against one of the cell surface proteins and flow cytometry used to compare the results with those obtained from affinity chromatography. Results: We have confirmed and extended the results obtained by Qureshi et al. In addition to the 44kD protein, we identified cell surface proteins with molecular weights of 98 and 106 kD binding with high affinity to both rsgp41 and rsgp36. We have demonstrated differences between human and murine cell lines in the expression of the cell surface proteins that interact with rsgp41 and rsgp36. Furthermore, a correlation between the level of rsgp41 and rsgp36 binding proteins, detected either by affinity chromatography or by reactivity with an antiserum directed against one of the cell surface binding components was shown. Conclusions: Three cell surface proteins, with molecular weights of 44, 98 and 106 kD, bind with high affinity to rs forms of gp41 and gp36. Their expression decreases from a T-lymphoid cell line, to a monoblastoid cell line, to a cell line representing mature monocytes. Human T-cell lymphotropic virus-infected cell lines show a predominance of the 44 kD protein. There are species-specific differences, in that murine cell lines lack the 44 kD protein.

Original languageEnglish
Pages (from-to)489-495
Number of pages7
JournalAIDS
Volume7
Issue number4
DOIs
StatePublished - 1993

Keywords

  • Putative HIV receptor
  • Transmembrane binding proteins
  • gp36
  • gp41

Fingerprint

Dive into the research topics of 'Cell surface proteins binding to recombinant soluble HIV-1 and HIV-2 transmembrane proteins'. Together they form a unique fingerprint.

Cite this