Cell segmentation using front vector flow guided active contours

Fuhai Li, Xiaobo Zhou, Hong Zhao, Stephen T.C. Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

20 Scopus citations

Abstract

Phase-contrast microscopy is a common approach for studying the dynamics of cell behaviors, such as cell migration. Cell segmentation is the basis of quantitative analysis of the immense cellular images. However, the complicated cell morphological appearance in phase-contrast microscopy images challenges the existing segmentation methods. This paper proposes a new cell segmentation method for cancer cell migration studies using phase-contrast images. Instead of segmenting cells directly based on commonly used low-level features, e.g. intensity and gradient, we first identify the leading protrusions, a high level feature, of cancer cells. Based on the identified cell leading protrusions, we introduce a front vector flow guided active contour, which guides the initial cell boundaries to the real boundaries. The experimental validation on a set of breast cancer cell images shows that the proposed method demonstrates fast, stable, and accurate segmentation for breast cancer cells with wide range of sizes and shapes.

Original languageEnglish
Title of host publicationMedical Image Computing and Computer-Assisted Intervention - MICCAI2009 - 12th International Conference, Proceedings
Pages609-616
Number of pages8
EditionPART 2
DOIs
StatePublished - Dec 1 2009
Externally publishedYes
Event12th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2009 - London, United Kingdom
Duration: Sep 20 2009Sep 24 2009

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
NumberPART 2
Volume5762 LNCS
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

Conference12th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2009
CountryUnited Kingdom
CityLondon
Period09/20/0909/24/09

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    Li, F., Zhou, X., Zhao, H., & Wong, S. T. C. (2009). Cell segmentation using front vector flow guided active contours. In Medical Image Computing and Computer-Assisted Intervention - MICCAI2009 - 12th International Conference, Proceedings (PART 2 ed., pp. 609-616). (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); Vol. 5762 LNCS, No. PART 2). https://doi.org/10.1007/978-3-642-04271-3_74