Cell penetrating recombinant Foxp3 protein enhances Treg function and ameliorates arthritis

Kentaro Yomogida, Shili Wu, Bobby Baravati, Camilo Avendano, Tom Caldwell, Brian Maniaci, Yong Zhu, Cong Qiu Chu

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Foxp3 is the master transcription factor for T regulatory (Treg) cell differentiation and function. This study aimed to test the therapeutic potential of cell penetrating recombinant Foxp3 protein in arthritis. Recombinant Foxp3 protein was fused to a cell penetrating polyarginine (Foxp3-11R) tag to facilitate intracellular transduction. In vitro Foxp3-11R treated CD4+ T cells showed a 50% increase in suppressive function compared with control protein treated cells. Severity of arthritis in Foxp3-11R treated mice was significantly reduced compared with those treated with a control protein. CD4+ T cells of lymph nodes and spleen from Foxp3-11R treated mice showed increased levels of Foxp3 expression compared with those of a control protein treated. These results demonstrated that Foxp3-11R can enhance T cell suppressive function and ameliorate experimental arthritis and suggest that cell penetrating recombinant Foxp3 is a potentially useful agent in therapy of arthritis.

Original languageEnglish
Pages (from-to)263-267
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume434
Issue number2
DOIs
StatePublished - May 3 2013
Externally publishedYes

Keywords

  • Arthritis
  • Foxp3
  • T regulatory cells

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