Cell penetrating recombinant Foxp3 protein enhances Treg function and ameliorates arthritis

Kentaro Yomogida; Shili Wu; Bobby Baravati; Camilo Avendano; Tom Caldwell; Brian Maniaci; Yong Zhu; Cong Qiu Chu

doi:10.1016/j.bbrc.2013.02.114

Cell penetrating recombinant Foxp3 protein enhances Treg function and ameliorates arthritis

Kentaro Yomogida, Shili Wu, Bobby Baravati, Camilo Avendano, Tom Caldwell, Brian Maniaci, Yong Zhu, Cong Qiu Chu

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Foxp3 is the master transcription factor for T regulatory (Treg) cell differentiation and function. This study aimed to test the therapeutic potential of cell penetrating recombinant Foxp3 protein in arthritis. Recombinant Foxp3 protein was fused to a cell penetrating polyarginine (Foxp3-11R) tag to facilitate intracellular transduction. In vitro Foxp3-11R treated CD4⁺ T cells showed a 50% increase in suppressive function compared with control protein treated cells. Severity of arthritis in Foxp3-11R treated mice was significantly reduced compared with those treated with a control protein. CD4⁺ T cells of lymph nodes and spleen from Foxp3-11R treated mice showed increased levels of Foxp3 expression compared with those of a control protein treated. These results demonstrated that Foxp3-11R can enhance T cell suppressive function and ameliorate experimental arthritis and suggest that cell penetrating recombinant Foxp3 is a potentially useful agent in therapy of arthritis.

Original language	English
Pages (from-to)	263-267
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	434
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2013.02.114
State	Published - May 3 2013

Keywords

Arthritis
Foxp3
T regulatory cells

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title = "Cell penetrating recombinant Foxp3 protein enhances Treg function and ameliorates arthritis",

abstract = "Foxp3 is the master transcription factor for T regulatory (Treg) cell differentiation and function. This study aimed to test the therapeutic potential of cell penetrating recombinant Foxp3 protein in arthritis. Recombinant Foxp3 protein was fused to a cell penetrating polyarginine (Foxp3-11R) tag to facilitate intracellular transduction. In vitro Foxp3-11R treated CD4+ T cells showed a 50% increase in suppressive function compared with control protein treated cells. Severity of arthritis in Foxp3-11R treated mice was significantly reduced compared with those treated with a control protein. CD4+ T cells of lymph nodes and spleen from Foxp3-11R treated mice showed increased levels of Foxp3 expression compared with those of a control protein treated. These results demonstrated that Foxp3-11R can enhance T cell suppressive function and ameliorate experimental arthritis and suggest that cell penetrating recombinant Foxp3 is a potentially useful agent in therapy of arthritis.",

keywords = "Arthritis, Foxp3, T regulatory cells",

author = "Kentaro Yomogida and Shili Wu and Bobby Baravati and Camilo Avendano and Tom Caldwell and Brian Maniaci and Yong Zhu and Chu, {Cong Qiu}",

note = "Funding Information: This work was supported by a grant from NIH to CQC ( AR055254 ) and Portland VA Medical Center to CQC.",

year = "2013",

month = may,

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doi = "10.1016/j.bbrc.2013.02.114",

language = "English",

volume = "434",

pages = "263--267",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Cell penetrating recombinant Foxp3 protein enhances Treg function and ameliorates arthritis

AU - Yomogida, Kentaro

AU - Wu, Shili

AU - Baravati, Bobby

AU - Avendano, Camilo

AU - Caldwell, Tom

AU - Maniaci, Brian

AU - Zhu, Yong

AU - Chu, Cong Qiu

N1 - Funding Information: This work was supported by a grant from NIH to CQC ( AR055254 ) and Portland VA Medical Center to CQC.

PY - 2013/5/3

Y1 - 2013/5/3

N2 - Foxp3 is the master transcription factor for T regulatory (Treg) cell differentiation and function. This study aimed to test the therapeutic potential of cell penetrating recombinant Foxp3 protein in arthritis. Recombinant Foxp3 protein was fused to a cell penetrating polyarginine (Foxp3-11R) tag to facilitate intracellular transduction. In vitro Foxp3-11R treated CD4+ T cells showed a 50% increase in suppressive function compared with control protein treated cells. Severity of arthritis in Foxp3-11R treated mice was significantly reduced compared with those treated with a control protein. CD4+ T cells of lymph nodes and spleen from Foxp3-11R treated mice showed increased levels of Foxp3 expression compared with those of a control protein treated. These results demonstrated that Foxp3-11R can enhance T cell suppressive function and ameliorate experimental arthritis and suggest that cell penetrating recombinant Foxp3 is a potentially useful agent in therapy of arthritis.

AB - Foxp3 is the master transcription factor for T regulatory (Treg) cell differentiation and function. This study aimed to test the therapeutic potential of cell penetrating recombinant Foxp3 protein in arthritis. Recombinant Foxp3 protein was fused to a cell penetrating polyarginine (Foxp3-11R) tag to facilitate intracellular transduction. In vitro Foxp3-11R treated CD4+ T cells showed a 50% increase in suppressive function compared with control protein treated cells. Severity of arthritis in Foxp3-11R treated mice was significantly reduced compared with those treated with a control protein. CD4+ T cells of lymph nodes and spleen from Foxp3-11R treated mice showed increased levels of Foxp3 expression compared with those of a control protein treated. These results demonstrated that Foxp3-11R can enhance T cell suppressive function and ameliorate experimental arthritis and suggest that cell penetrating recombinant Foxp3 is a potentially useful agent in therapy of arthritis.

KW - Arthritis

KW - Foxp3

KW - T regulatory cells

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U2 - 10.1016/j.bbrc.2013.02.114

DO - 10.1016/j.bbrc.2013.02.114

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SN - 0006-291X

VL - 434

SP - 263

EP - 267

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Cell penetrating recombinant Foxp3 protein enhances Treg function and ameliorates arthritis

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Keywords

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