Cell morphology and migration of nucleus pulposus cells depends on substrate stiffness and ligand

Priscilla Y. Hwang; Christopher L. Gilchrist; Aubrey T. Francisco; Jun Chen; Lori A. Setton

doi:10.1115/SBC2012-80338

Cell morphology and migration of nucleus pulposus cells depends on substrate stiffness and ligand

Priscilla Y. Hwang, Christopher L. Gilchrist, Aubrey T. Francisco, Jun Chen, Lori A. Setton

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Changes in nucleus pulposus (NP) cell phenotype and morphology are implicated in the progression of intervertebral disc (IVD) disorders. Understanding how changes in the NP cell microenvironment influence cell behavior and function is important for revealing how pathology-related changes in IVD extracellular matrix may affect NP cell biology. In this study, live-cell imaging techniques were utilized to study changes in cell migration and morphology when cultured upon substrates of different matrix proteins and stiffnesses. Results indicate that soft substrates containing matrix proteins promote cell clustering and cell-cell interactions which mimic in vivo conditions of healthy NP cells.

Original language	English
Title of host publication	ASME 2012 Summer Bioengineering Conference, SBC 2012
Pages	1205-1206
Number of pages	2
DOIs	https://doi.org/10.1115/SBC2012-80338
State	Published - 2012
Event	ASME 2012 Summer Bioengineering Conference, SBC 2012 - Fajardo, Puerto Rico Duration: Jun 20 2012 → Jun 23 2012

Publication series

Name	ASME 2012 Summer Bioengineering Conference, SBC 2012

Conference

Conference	ASME 2012 Summer Bioengineering Conference, SBC 2012
Country/Territory	Puerto Rico
City	Fajardo
Period	06/20/12 → 06/23/12

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10.1115/SBC2012-80338

Cite this

@inproceedings{dab548a265034c9e94e1f0616ae95d20,

title = "Cell morphology and migration of nucleus pulposus cells depends on substrate stiffness and ligand",

abstract = "Changes in nucleus pulposus (NP) cell phenotype and morphology are implicated in the progression of intervertebral disc (IVD) disorders. Understanding how changes in the NP cell microenvironment influence cell behavior and function is important for revealing how pathology-related changes in IVD extracellular matrix may affect NP cell biology. In this study, live-cell imaging techniques were utilized to study changes in cell migration and morphology when cultured upon substrates of different matrix proteins and stiffnesses. Results indicate that soft substrates containing matrix proteins promote cell clustering and cell-cell interactions which mimic in vivo conditions of healthy NP cells.",

author = "Hwang, {Priscilla Y.} and Gilchrist, {Christopher L.} and Francisco, {Aubrey T.} and Jun Chen and Setton, {Lori A.}",

year = "2012",

doi = "10.1115/SBC2012-80338",

language = "English",

isbn = "9780791844809",

series = "ASME 2012 Summer Bioengineering Conference, SBC 2012",

pages = "1205--1206",

booktitle = "ASME 2012 Summer Bioengineering Conference, SBC 2012",

note = "ASME 2012 Summer Bioengineering Conference, SBC 2012 ; Conference date: 20-06-2012 Through 23-06-2012",

}

Hwang, PY, Gilchrist, CL, Francisco, AT, Chen, J & Setton, LA 2012, Cell morphology and migration of nucleus pulposus cells depends on substrate stiffness and ligand. in ASME 2012 Summer Bioengineering Conference, SBC 2012. ASME 2012 Summer Bioengineering Conference, SBC 2012, pp. 1205-1206, ASME 2012 Summer Bioengineering Conference, SBC 2012, Fajardo, Puerto Rico, 06/20/12. https://doi.org/10.1115/SBC2012-80338

Cell morphology and migration of nucleus pulposus cells depends on substrate stiffness and ligand. / Hwang, Priscilla Y.; Gilchrist, Christopher L.; Francisco, Aubrey T. et al.
ASME 2012 Summer Bioengineering Conference, SBC 2012. 2012. p. 1205-1206 (ASME 2012 Summer Bioengineering Conference, SBC 2012).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Cell morphology and migration of nucleus pulposus cells depends on substrate stiffness and ligand

AU - Hwang, Priscilla Y.

AU - Gilchrist, Christopher L.

AU - Francisco, Aubrey T.

AU - Chen, Jun

AU - Setton, Lori A.

PY - 2012

Y1 - 2012

N2 - Changes in nucleus pulposus (NP) cell phenotype and morphology are implicated in the progression of intervertebral disc (IVD) disorders. Understanding how changes in the NP cell microenvironment influence cell behavior and function is important for revealing how pathology-related changes in IVD extracellular matrix may affect NP cell biology. In this study, live-cell imaging techniques were utilized to study changes in cell migration and morphology when cultured upon substrates of different matrix proteins and stiffnesses. Results indicate that soft substrates containing matrix proteins promote cell clustering and cell-cell interactions which mimic in vivo conditions of healthy NP cells.

AB - Changes in nucleus pulposus (NP) cell phenotype and morphology are implicated in the progression of intervertebral disc (IVD) disorders. Understanding how changes in the NP cell microenvironment influence cell behavior and function is important for revealing how pathology-related changes in IVD extracellular matrix may affect NP cell biology. In this study, live-cell imaging techniques were utilized to study changes in cell migration and morphology when cultured upon substrates of different matrix proteins and stiffnesses. Results indicate that soft substrates containing matrix proteins promote cell clustering and cell-cell interactions which mimic in vivo conditions of healthy NP cells.

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U2 - 10.1115/SBC2012-80338

DO - 10.1115/SBC2012-80338

M3 - Conference contribution

AN - SCOPUS:84882723061

SN - 9780791844809

T3 - ASME 2012 Summer Bioengineering Conference, SBC 2012

SP - 1205

EP - 1206

BT - ASME 2012 Summer Bioengineering Conference, SBC 2012

T2 - ASME 2012 Summer Bioengineering Conference, SBC 2012

Y2 - 20 June 2012 through 23 June 2012

ER -

Cell morphology and migration of nucleus pulposus cells depends on substrate stiffness and ligand

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