TY - JOUR
T1 - cDNA microarray analysis of adapting bowel after intestinal resection
AU - Stern, L. E.
AU - Erwin, C. R.
AU - Falcone, Jr
AU - Huang, F. S.
AU - Kemp, C. J.
AU - Williams, J. L.
AU - Warner, B. W.
N1 - Funding Information:
Supported by National Institutes of Health F32 DK09882 (LES), National Institutes of Health RO-1 DK53234 (BWW) and a grant from the Children's Hospital Campaign for Children Fund, Children's Hospital Medical Center, Cincinnati, OH.
PY - 2001
Y1 - 2001
N2 - Background/Purpose: Studies of the genetic regulation of various physiologic processes have been hampered by methodologies that are limited to the analysis of individual genes. The advent of cDNA microarray technology has permitted the simultaneous screening of numerous genes for alterations in expression. In this study, cDNA microarrays were used to evaluate gene expression changes during the intestinal adaptive response to massive small bowel resection (SBR). Methods: Male ICR mice (n = 20) underwent either a 50% SBR or sham operation and then were given either orogastric epidermal growth factor (EGF, 50 μg/kg/d) or saline. After 3 days, cDNA microarray analysis was performed on mRNA extracted from the remnant ileum. Results: From over 8,700 different genes, the array identified 27 genes that were altered 2-fold or greater after SBR. Small proline-rich protein 2 (sprr2), the gene with the greatest expression change (4.9-fold), was further upregulated by EGF. This gene has never been characterized in the intestine or described in intestinal adaptation. Conclusions: cDNA microarray analysis showed enhanced expression of sprr2, a gene not previously known to be involved in the physiology of adaptation after SBR. This technology provides a more rapid and efficient means of dissecting the complex genetic regulation of gut adaptation.
AB - Background/Purpose: Studies of the genetic regulation of various physiologic processes have been hampered by methodologies that are limited to the analysis of individual genes. The advent of cDNA microarray technology has permitted the simultaneous screening of numerous genes for alterations in expression. In this study, cDNA microarrays were used to evaluate gene expression changes during the intestinal adaptive response to massive small bowel resection (SBR). Methods: Male ICR mice (n = 20) underwent either a 50% SBR or sham operation and then were given either orogastric epidermal growth factor (EGF, 50 μg/kg/d) or saline. After 3 days, cDNA microarray analysis was performed on mRNA extracted from the remnant ileum. Results: From over 8,700 different genes, the array identified 27 genes that were altered 2-fold or greater after SBR. Small proline-rich protein 2 (sprr2), the gene with the greatest expression change (4.9-fold), was further upregulated by EGF. This gene has never been characterized in the intestine or described in intestinal adaptation. Conclusions: cDNA microarray analysis showed enhanced expression of sprr2, a gene not previously known to be involved in the physiology of adaptation after SBR. This technology provides a more rapid and efficient means of dissecting the complex genetic regulation of gut adaptation.
KW - Epidermal growth factor
KW - Intestinal adaptation
KW - Short bowel syndrome
KW - Small proline-rich protein
KW - cDNA microarray
UR - http://www.scopus.com/inward/record.url?scp=0035172488&partnerID=8YFLogxK
U2 - 10.1053/jpsu.2001.20050
DO - 10.1053/jpsu.2001.20050
M3 - Article
C2 - 11150463
AN - SCOPUS:0035172488
SN - 0022-3468
VL - 36
SP - 190
EP - 195
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
IS - 1
ER -