Cdh1-APC Controls Axonal Growth and Patterning in the Mammalian Brain

Yoshiyuki Konishi; Judith Stegmüller; Takahiko Matsuda; Shirin Bonni; Azad Bonni

doi:10.1126/science.1093712

Cdh1-APC Controls Axonal Growth and Patterning in the Mammalian Brain

Yoshiyuki Konishi, Judith Stegmüller, Takahiko Matsuda, Shirin Bonni, Azad Bonni

Department of Neuroscience

Research output: Contribution to journal › Article › peer-review

330 Scopus citations

Abstract

The anaphase-promoting complex (APC) is highly expressed in postmitotic neurons, but its function in the nervous system was previously unknown. We report that the inhibition of Cdh1-APC in primary neurons specifically enhanced axonal growth. Cdh1 knockdown in cerebellar slice overlay assays and in the developing rat cerebellum in vivo revealed cell-autonomous abnormalities in layer-specific growth of granule neuron axons and parallel fiber patterning. Cdh1 RNA interference in neurons was also found to override the inhibitory influence of myelin on axonal growth. Thus, Cdh1-APC appears to play a role in regulating axonal growth and patterning in the developing brain that may also limit the growth of injured axons in the adult brain.

Original language	English
Pages (from-to)	1026-1030
Number of pages	5
Journal	Science
Volume	303
Issue number	5660
DOIs	https://doi.org/10.1126/science.1093712
State	Published - Feb 13 2004

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abstract = "The anaphase-promoting complex (APC) is highly expressed in postmitotic neurons, but its function in the nervous system was previously unknown. We report that the inhibition of Cdh1-APC in primary neurons specifically enhanced axonal growth. Cdh1 knockdown in cerebellar slice overlay assays and in the developing rat cerebellum in vivo revealed cell-autonomous abnormalities in layer-specific growth of granule neuron axons and parallel fiber patterning. Cdh1 RNA interference in neurons was also found to override the inhibitory influence of myelin on axonal growth. Thus, Cdh1-APC appears to play a role in regulating axonal growth and patterning in the developing brain that may also limit the growth of injured axons in the adult brain.",

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AU - Bonni, Azad

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AB - The anaphase-promoting complex (APC) is highly expressed in postmitotic neurons, but its function in the nervous system was previously unknown. We report that the inhibition of Cdh1-APC in primary neurons specifically enhanced axonal growth. Cdh1 knockdown in cerebellar slice overlay assays and in the developing rat cerebellum in vivo revealed cell-autonomous abnormalities in layer-specific growth of granule neuron axons and parallel fiber patterning. Cdh1 RNA interference in neurons was also found to override the inhibitory influence of myelin on axonal growth. Thus, Cdh1-APC appears to play a role in regulating axonal growth and patterning in the developing brain that may also limit the growth of injured axons in the adult brain.

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Cdh1-APC Controls Axonal Growth and Patterning in the Mammalian Brain

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