CD8+ T Cells Orchestrate pDC-XCR1+ Dendritic Cell Spatial and Functional Cooperativity to Optimize Priming

Anna Brewitz, Sarah Eickhoff, Sabrina Dähling, Thomas Quast, Sammy Bedoui, Richard A. Kroczek, Christian Kurts, Natalio Garbi, Winfried Barchet, Matteo Iannacone, Frederick Klauschen, Waldemar Kolanus, Tsuneyasu Kaisho, Marco Colonna, Ronald N. Germain, Wolfgang Kastenmüller

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Adaptive cellular immunity is initiated by antigen-specific interactions between T lymphocytes and dendritic cells (DCs). Plasmacytoid DCs (pDCs) support antiviral immunity by linking innate and adaptive immune responses. Here we examined pDC spatiotemporal dynamics during viral infection to uncover when, where, and how they exert their functions. We found that pDCs accumulated at sites of CD8+ T cell antigen-driven activation in a CCR5-dependent fashion. Furthermore, activated CD8+ T cells orchestrated the local recruitment of lymph node-resident XCR1 chemokine receptor-expressing DCs via secretion of the XCL1 chemokine. Functionally, this CD8+ T cell-mediated reorganization of the local DC network allowed for the interaction and cooperation of pDCs and XCR1+ DCs, thereby optimizing XCR1+ DC maturation and cross-presentation. These data support a model in which CD8+ T cells upon activation create their own optimal priming microenvironment by recruiting additional DC subsets to the site of initial antigen recognition.

Original languageEnglish
Pages (from-to)205-219
Number of pages15
JournalImmunity
Volume46
Issue number2
DOIs
StatePublished - Feb 21 2017

Keywords

  • CCL3
  • CCL4
  • CCR5
  • CXCR3
  • XCL1
  • chemokine
  • cooperation
  • migration
  • spatiotemporal
  • viral infection

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    Brewitz, A., Eickhoff, S., Dähling, S., Quast, T., Bedoui, S., Kroczek, R. A., Kurts, C., Garbi, N., Barchet, W., Iannacone, M., Klauschen, F., Kolanus, W., Kaisho, T., Colonna, M., Germain, R. N., & Kastenmüller, W. (2017). CD8+ T Cells Orchestrate pDC-XCR1+ Dendritic Cell Spatial and Functional Cooperativity to Optimize Priming. Immunity, 46(2), 205-219. https://doi.org/10.1016/j.immuni.2017.01.003