@article{14d09af1cc804d76851144d5eb1b51fa,
title = "CD8+ T Cells Orchestrate pDC-XCR1+ Dendritic Cell Spatial and Functional Cooperativity to Optimize Priming",
abstract = "Adaptive cellular immunity is initiated by antigen-specific interactions between T lymphocytes and dendritic cells (DCs). Plasmacytoid DCs (pDCs) support antiviral immunity by linking innate and adaptive immune responses. Here we examined pDC spatiotemporal dynamics during viral infection to uncover when, where, and how they exert their functions. We found that pDCs accumulated at sites of CD8+ T cell antigen-driven activation in a CCR5-dependent fashion. Furthermore, activated CD8+ T cells orchestrated the local recruitment of lymph node-resident XCR1 chemokine receptor-expressing DCs via secretion of the XCL1 chemokine. Functionally, this CD8+ T cell-mediated reorganization of the local DC network allowed for the interaction and cooperation of pDCs and XCR1+ DCs, thereby optimizing XCR1+ DC maturation and cross-presentation. These data support a model in which CD8+ T cells upon activation create their own optimal priming microenvironment by recruiting additional DC subsets to the site of initial antigen recognition.",
keywords = "CCL3, CCL4, CCR5, CXCR3, XCL1, chemokine, cooperation, migration, spatiotemporal, viral infection",
author = "Anna Brewitz and Sarah Eickhoff and Sabrina D{\"a}hling and Thomas Quast and Sammy Bedoui and Kroczek, {Richard A.} and Christian Kurts and Natalio Garbi and Winfried Barchet and Matteo Iannacone and Frederick Klauschen and Waldemar Kolanus and Tsuneyasu Kaisho and Marco Colonna and Germain, {Ronald N.} and Wolfgang Kastenm{\"u}ller",
note = "Funding Information: We would like to thank S. Ebbinghaus and S. Rathmann for technical assistance and D.H. Busch for kindly providing MHCI multimers. This research was supported by the Intramural Research Program, NIAID, NIH. W. Kastenm{\"u}ller, N.G., C.K., and W. Kolanus are members of the DFG Excellence Cluster ImmunoSensation in Bonn, Germany, and are supported by grant SFB704. S.D., S.B., and W. Kastenm{\"u}ller are supported by the DFG Graduate program 2168/1 (Bo&MeRang). W. Kastenm{\"u}ller is supported by NRW-R{\"u}ckkehrerprogramm of the German state of Northrhine-Westfalia. T.K. was supported by the Kishimoto Foundation and a Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science (JSPS, 26293106). F.K. is supported by the Einstein Foundation Berlin (JF-Klauschen-2014). Publisher Copyright: {\textcopyright} 2017",
year = "2017",
month = feb,
day = "21",
doi = "10.1016/j.immuni.2017.01.003",
language = "English",
volume = "46",
pages = "205--219",
journal = "Immunity",
issn = "1074-7613",
number = "2",
}