CD8α+ Dendritic Cells Are an Obligate Cellular Entry Point for Productive Infection by Listeria monocytogenes

Brian T. Edelson, Tara R. Bradstreet, Kai Hildner, Javier A. Carrero, Katherine E. Frederick, K. C. Wumesh, Roger Belizaire, Taiki Aoshi, Robert D. Schreiber, Mark J. Miller, Theresa L. Murphy, Emil R. Unanue, Kenneth M. Murphy

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

CD8α+ dendritic cells (DCs) prime cytotoxic T lymphocytes during viral infections and produce interleukin-12 in response to pathogens. Although the loss of CD8α+ DCs in Batf3-/- mice increases their susceptibility to several pathogens, we observed that Batf3-/- mice exhibited enhanced resistance to the intracellular bacterium Listeria monocytogenes. In wild-type mice, Listeria organisms, initially located in the splenic marginal zone, migrated to the periarteriolar lymphoid sheath (PALS) where they grew exponentially and induced widespread lymphocyte apoptosis. In Batf3-/- mice, however, Listeria organisms remain trapped in the marginal zone, failed to traffic into the PALS, and were rapidly cleared by phagocytes. In addition, Batf3-/- mice, which lacked the normal population of hepatic CD103+ peripheral DCs, also showed protection from liver infection. These results suggest that Batf3-dependent CD8α+ and CD103+ DCs provide initial cellular entry points within the reticuloendothelial system by which Listeria establishes productive infection.

Original languageEnglish
Pages (from-to)236-248
Number of pages13
JournalImmunity
Volume35
Issue number2
DOIs
StatePublished - Aug 26 2011

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