CD4+25+ regulatory T cells limit Th1-autoimmunity by inducing IL-10 producing T cells following human lung transplantation

A. Bharat, R. C. Fields, N. Steward, E. P. Trulock, G. A. Patterson, T. Mohanakumar

Research output: Contribution to journalArticlepeer-review

97 Scopus citations


Chronic human lung allograft rejection is manifested by bronchiolitis obliterans syndrome (BOS). BOS has a multifactorial etiology. Previous studies have indicated that both cellular and humoral alloimmunity play a significant role in the pathogenesis of BOS. Recently, autoimmunity has also been demonstrated to contribute to lung allograft rejection in animal models. However, the significance of autoimmunity in BOS remains unknown. In this report, we investigated the role of naturally occurring CD4+CD25+ regulatory T cells (T-regs) in modulating cellular autoimmunity to collagen type V (col-V), a 'sequestered' yet immunogenic self-protein present in the lung tissue, following lung transplantation (LT). We demonstrated that col-V reactive CD4+ T cells could be detected in the peripheral blood of lung transplant recipients. There was a predominance of IL-10 producing T cells (TIL-10) reactive to col-V with significantly lower levels of IFN-γ and IL-2 producing T cells (Th1 cells). The col-V specific TIL-10 cells suppressed the proliferation and expansion of col-V specific Th1 cells by IL-10-dependent and contact-independent pathways. The TIL-10 cells were distinct but their development was dependent on the presence of T-regs. Furthermore, during chronic lung allograft rejection there was a significant decline of T IL-10 cells with concomitant expansion of col-V-specific IFN-γ producing Th1 cells.

Original languageEnglish
Pages (from-to)1799-1808
Number of pages10
JournalAmerican Journal of Transplantation
Issue number8
StatePublished - Aug 2006


  • Autoimmunity
  • Bronchiolitis obliterans
  • Collagen type V
  • IL-10
  • Lung transplant
  • Regulatory T cells


Dive into the research topics of 'CD4+25+ regulatory T cells limit Th1-autoimmunity by inducing IL-10 producing T cells following human lung transplantation'. Together they form a unique fingerprint.

Cite this