TY - JOUR
T1 - CD36 is indispensable for thermogenesis under conditions of fasting and cold stress
AU - Putri, Mirasari
AU - Syamsunarno, Mas Rizky A.A.
AU - Iso, Tatsuya
AU - Yamaguchi, Aiko
AU - Hanaoka, Hirofumi
AU - Sunaga, Hiroaki
AU - Koitabashi, Norimichi
AU - Matsui, Hiroki
AU - Yamazaki, Chiho
AU - Kameo, Satomi
AU - Tsushima, Yoshito
AU - Yokoyama, Tomoyuki
AU - Koyama, Hiroshi
AU - Abumrad, Nada A.
AU - Kurabayashi, Masahiko
N1 - Funding Information:
We thank Miki Matsui, Yukiyo Tosaka, Keiko Matsukura and Takako Kobayashi for excellent technical help. This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the promotion of Science , to T.I. ( 26461123 ), to M.K. ( 24390194 ). a grant from the Japan Cardiovascular Foundation (to MK) and a grant from the Vehicle Racing Commemorative Foundation and (to TI).
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/2/20
Y1 - 2015/2/20
N2 - Hypothermia can occur during fasting when thermoregulatory mechanisms, involving fatty acid (FA) utilization, are disturbed. CD36/FA translocase is a membrane protein which facilitates membrane transport of long-chain FA in the FA consuming heart, skeletal muscle (SkM) and adipose tissues. It also accelerates uptake of triglyceride-rich lipoprotein by brown adipose tissue (BAT) in a cold environment. In mice deficient for CD36 (CD36-/- mice), FA uptake is markedly reduced with a compensatory increase in glucose uptake in the heart and SkM, resulting in lower levels of blood glucose especially during fasting. However, the role of CD36 in thermogenic activity during fasting remains to be determined. In fasted CD36-/- mice, body temperature drastically decreased shortly after cold exposure. The hypothermia was accompanied by a marked reduction in blood glucose and in stores of triacylglycerols in BAT and of glycogen in glycolytic SkM. Biodistribution analysis using the FA analogue 125I-BMIPP and the glucose analogue 18F-FDG revealed that uptake of FA and glucose was severely impaired in BAT and glycolytic SkM in cold-exposed CD36-/- mice. Further, induction of the genes of thermogenesis in BAT was blunted in fasted CD36-/- mice after cold exposure. These findings strongly suggest that CD36-/- mice exhibit pronounced hypothermia after fasting due to depletion of energy storage in BAT and glycolytic SkM and to reduced supply of energy substrates to these tissues. Our study underscores the importance of CD36 for nutrient homeostasis to survive potentially life-threatening challenges, such as cold and starvation.
AB - Hypothermia can occur during fasting when thermoregulatory mechanisms, involving fatty acid (FA) utilization, are disturbed. CD36/FA translocase is a membrane protein which facilitates membrane transport of long-chain FA in the FA consuming heart, skeletal muscle (SkM) and adipose tissues. It also accelerates uptake of triglyceride-rich lipoprotein by brown adipose tissue (BAT) in a cold environment. In mice deficient for CD36 (CD36-/- mice), FA uptake is markedly reduced with a compensatory increase in glucose uptake in the heart and SkM, resulting in lower levels of blood glucose especially during fasting. However, the role of CD36 in thermogenic activity during fasting remains to be determined. In fasted CD36-/- mice, body temperature drastically decreased shortly after cold exposure. The hypothermia was accompanied by a marked reduction in blood glucose and in stores of triacylglycerols in BAT and of glycogen in glycolytic SkM. Biodistribution analysis using the FA analogue 125I-BMIPP and the glucose analogue 18F-FDG revealed that uptake of FA and glucose was severely impaired in BAT and glycolytic SkM in cold-exposed CD36-/- mice. Further, induction of the genes of thermogenesis in BAT was blunted in fasted CD36-/- mice after cold exposure. These findings strongly suggest that CD36-/- mice exhibit pronounced hypothermia after fasting due to depletion of energy storage in BAT and glycolytic SkM and to reduced supply of energy substrates to these tissues. Our study underscores the importance of CD36 for nutrient homeostasis to survive potentially life-threatening challenges, such as cold and starvation.
KW - Brown adipose tissue
KW - CD36
KW - Fatty acid
KW - Hypothermia
KW - Skeletal muscle
UR - http://www.scopus.com/inward/record.url?scp=84922738113&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2014.12.124
DO - 10.1016/j.bbrc.2014.12.124
M3 - Article
C2 - 25596128
AN - SCOPUS:84922738113
SN - 0006-291X
VL - 457
SP - 520
EP - 525
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -