CD301b+ dendritic cells stimulate tissue-resident memory CD8+ T cells to protect against genital HSV-2

Haina Shin; Yosuke Kumamoto; Smita Gopinath; Akiko Iwasaki

doi:10.1038/ncomms13346

CD301b+ dendritic cells stimulate tissue-resident memory CD8+ T cells to protect against genital HSV-2

Haina Shin
, Yosuke Kumamoto
, Smita Gopinath
, Akiko Iwasaki

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

Tissue-resident memory CD8+ T (CD8 T_RM) cells are an essential component of protective immune responses at barrier tissues, including the female genital tract. However, the mechanisms that lead to the initiation of CD8 T_RM -mediated protective immunity after viral infection are unclear. Here we report that CD8 T_RM cells established by 'prime and pull' method confer protection against genital HSV-2 infection, and that IFN-γ produced by CD8 T_RM cells is required for this protection. Furthermore, we find that CD8 T_RM -cell restimulation depends on a population of CD301b⁺ antigen-presenting cells (APC) in the lamina propria. Elimination of MHC class I on CD301b⁺ dendritic cells abrogates protective immunity, suggesting the requirement for cognate antigen presentation to CD8 T_RM cells by CD301b⁺ dendritic cells. These results define the requirements for CD8 T_RM cells in protection against genital HSV-2 infection and identify the population of APC that are responsible for activating these cells.

Original language	English
Article number	13346
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms13346
State	Published - Nov 9 2016

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title = "CD301b+ dendritic cells stimulate tissue-resident memory CD8+ T cells to protect against genital HSV-2",

abstract = "Tissue-resident memory CD8+ T (CD8 TRM) cells are an essential component of protective immune responses at barrier tissues, including the female genital tract. However, the mechanisms that lead to the initiation of CD8 TRM -mediated protective immunity after viral infection are unclear. Here we report that CD8 TRM cells established by 'prime and pull' method confer protection against genital HSV-2 infection, and that IFN-γ produced by CD8 TRM cells is required for this protection. Furthermore, we find that CD8 TRM -cell restimulation depends on a population of CD301b+ antigen-presenting cells (APC) in the lamina propria. Elimination of MHC class I on CD301b+ dendritic cells abrogates protective immunity, suggesting the requirement for cognate antigen presentation to CD8 TRM cells by CD301b+ dendritic cells. These results define the requirements for CD8 TRM cells in protection against genital HSV-2 infection and identify the population of APC that are responsible for activating these cells.",

author = "Haina Shin and Yosuke Kumamoto and Smita Gopinath and Akiko Iwasaki",

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T1 - CD301b+ dendritic cells stimulate tissue-resident memory CD8+ T cells to protect against genital HSV-2

AU - Shin, Haina

AU - Kumamoto, Yosuke

AU - Gopinath, Smita

AU - Iwasaki, Akiko

N1 - Publisher Copyright: © The Author(s) 2016.

PY - 2016/11/9

Y1 - 2016/11/9

N2 - Tissue-resident memory CD8+ T (CD8 TRM) cells are an essential component of protective immune responses at barrier tissues, including the female genital tract. However, the mechanisms that lead to the initiation of CD8 TRM -mediated protective immunity after viral infection are unclear. Here we report that CD8 TRM cells established by 'prime and pull' method confer protection against genital HSV-2 infection, and that IFN-γ produced by CD8 TRM cells is required for this protection. Furthermore, we find that CD8 TRM -cell restimulation depends on a population of CD301b+ antigen-presenting cells (APC) in the lamina propria. Elimination of MHC class I on CD301b+ dendritic cells abrogates protective immunity, suggesting the requirement for cognate antigen presentation to CD8 TRM cells by CD301b+ dendritic cells. These results define the requirements for CD8 TRM cells in protection against genital HSV-2 infection and identify the population of APC that are responsible for activating these cells.

AB - Tissue-resident memory CD8+ T (CD8 TRM) cells are an essential component of protective immune responses at barrier tissues, including the female genital tract. However, the mechanisms that lead to the initiation of CD8 TRM -mediated protective immunity after viral infection are unclear. Here we report that CD8 TRM cells established by 'prime and pull' method confer protection against genital HSV-2 infection, and that IFN-γ produced by CD8 TRM cells is required for this protection. Furthermore, we find that CD8 TRM -cell restimulation depends on a population of CD301b+ antigen-presenting cells (APC) in the lamina propria. Elimination of MHC class I on CD301b+ dendritic cells abrogates protective immunity, suggesting the requirement for cognate antigen presentation to CD8 TRM cells by CD301b+ dendritic cells. These results define the requirements for CD8 TRM cells in protection against genital HSV-2 infection and identify the population of APC that are responsible for activating these cells.

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DO - 10.1038/ncomms13346

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VL - 7

JO - Nature communications

JF - Nature communications

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ER -

CD301b+ dendritic cells stimulate tissue-resident memory CD8+ T cells to protect against genital HSV-2

Abstract

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