Abstract
Interleukin-17A (IL-17A) is a pro-inflammatory cytokine that has an important role at mucosal sites in a wide range of immune responses including infection, allergy and auto-immunity. γδ T cells are recognized as IL-17 producers, but based on the level of CD3 expression, we now define the remarkable ability of a CD3 bright γδ T-cell subset with an effector memory phenotype to rapidly produce IL-17A, but not interferon-γ. CD3 bright γδ T cells uniformly express the canonical germline encoded Vγ6/Vδ1+T-cell receptor. They are widely distributed with a preferential representation in the lungs and skin are negatively impacted in the absence of retinoic acid receptor-related orphan receptor gammat expression or endogenous flora. This population responded rapidly to various stimuli in a mechanism involving IL-23 and NOD-like receptor family, pyrin domain containing 3 (NLRP3)-inflammasome-dependent IL-1β. Finally, we demonstrated that IL-17-producing CD3 bright γδ T cells responded promptly and strongly to pneumococcal infection and during skin inflammation. Here, we propose a new way to specifically analyze IL-17-producing Vγ6/Vδ1+T cells based on the level of CD3 signals. Using this gating strategy, our data reinforce the crucial role of this γδ T-cell subset in respiratory and skin disorders.
Original language | English |
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Pages (from-to) | 198-212 |
Number of pages | 15 |
Journal | Immunology and Cell Biology |
Volume | 93 |
Issue number | 2 |
DOIs | |
State | Published - Feb 12 2015 |