CD3 bright signals on γδ T cells identify IL-17A-producing Vγ6Vδ1+T cells

C. Paget, M. T. Chow, N. A. Gherardin, P. A. Beavis, A. P. Uldrich, H. Duret, M. Hassane, F. Souza-Fonseca-Guimaraes, D. A. Mogilenko, D. Staumont-Sallé, N. K. Escalante, G. R. Hill, P. Neeson, D. S. Ritchie, D. Dombrowicz, T. Mallevaey, F. Trottein, G. T. Belz, D. I. Godfrey, M. J. Smyth

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


Interleukin-17A (IL-17A) is a pro-inflammatory cytokine that has an important role at mucosal sites in a wide range of immune responses including infection, allergy and auto-immunity. γδ T cells are recognized as IL-17 producers, but based on the level of CD3 expression, we now define the remarkable ability of a CD3 bright γδ T-cell subset with an effector memory phenotype to rapidly produce IL-17A, but not interferon-γ. CD3 bright γδ T cells uniformly express the canonical germline encoded Vγ6/Vδ1+T-cell receptor. They are widely distributed with a preferential representation in the lungs and skin are negatively impacted in the absence of retinoic acid receptor-related orphan receptor gammat expression or endogenous flora. This population responded rapidly to various stimuli in a mechanism involving IL-23 and NOD-like receptor family, pyrin domain containing 3 (NLRP3)-inflammasome-dependent IL-1β. Finally, we demonstrated that IL-17-producing CD3 bright γδ T cells responded promptly and strongly to pneumococcal infection and during skin inflammation. Here, we propose a new way to specifically analyze IL-17-producing Vγ6/Vδ1+T cells based on the level of CD3 signals. Using this gating strategy, our data reinforce the crucial role of this γδ T-cell subset in respiratory and skin disorders.

Original languageEnglish
Pages (from-to)198-212
Number of pages15
JournalImmunology and Cell Biology
Issue number2
StatePublished - Feb 12 2015


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