TY - JOUR
T1 - CD2AP localizes to the slit diaphragm and binds to nephrin via a novel C-terminal domain
AU - Shi, Neng Yao
AU - Li, Jun
AU - Cotran, Ramzi
AU - Mundel, Peter
AU - Miner, Jeffrey H.
AU - Shaw, Andrey S.
N1 - Funding Information:
Supported by grants from the National Institutes of Health to A. S. and J. M.
PY - 2001/12
Y1 - 2001/12
N2 - CD2AP, an adapter protein containing multiple SH3 domains, plays a critical role in kidney function. Mice lacking CD2AP die soon after birth because of kidney failure. In the kidney, CD2AP is expressed in glomerular podocytes, which suggests that it may play a role in a specialized adhesion complex known as the slit diaphragm. One of the major components of the slit diaphragm is nephrin, a podocyte-specific protein. Here we demonstrate that CD2AP localizes to the slit diaphragm in podocytes using immunoelectron microscopy and that nephrin and CD2AP co-immunoprecipitate from a podocyte cell line. The specificity of this interaction was verified by mapping studies, which demonstrated that a novel domain at the C terminus of CD2AP interacts with the C-terminal portion of the nephrin cytoplasmic domain. These studies lend further support to the idea that CD2AP plays a role in the structural integrity of the slit diaphragm.
AB - CD2AP, an adapter protein containing multiple SH3 domains, plays a critical role in kidney function. Mice lacking CD2AP die soon after birth because of kidney failure. In the kidney, CD2AP is expressed in glomerular podocytes, which suggests that it may play a role in a specialized adhesion complex known as the slit diaphragm. One of the major components of the slit diaphragm is nephrin, a podocyte-specific protein. Here we demonstrate that CD2AP localizes to the slit diaphragm in podocytes using immunoelectron microscopy and that nephrin and CD2AP co-immunoprecipitate from a podocyte cell line. The specificity of this interaction was verified by mapping studies, which demonstrated that a novel domain at the C terminus of CD2AP interacts with the C-terminal portion of the nephrin cytoplasmic domain. These studies lend further support to the idea that CD2AP plays a role in the structural integrity of the slit diaphragm.
UR - http://www.scopus.com/inward/record.url?scp=0035191415&partnerID=8YFLogxK
U2 - 10.1016/S0002-9440(10)63080-5
DO - 10.1016/S0002-9440(10)63080-5
M3 - Article
C2 - 11733379
AN - SCOPUS:0035191415
SN - 0002-9440
VL - 159
SP - 2303
EP - 2308
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 6
ER -