CCL3 promotes germinal center B cells sampling by follicular regulatory T cells in murine lymph nodes

Zachary L. Benet; Matangi Marthi; Fang Ke; Rita Wu; Jackson S. Turner; Jahan B. Gabayre; Michael I. Ivanitskiy; Sahil S. Sethi; Irina L. Grigorova

doi:10.3389/fimmu.2018.02044

CCL3 promotes germinal center B cells sampling by follicular regulatory T cells in murine lymph nodes

Zachary L. Benet
, Matangi Marthi
, Fang Ke
, Rita Wu
, Jackson S. Turner
, Jahan B. Gabayre
, Michael I. Ivanitskiy
, Sahil S. Sethi
, Irina L. Grigorova

Division of Immunobiology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.

Original language	English
Article number	2044
Journal	Frontiers in immunology
Volume	9
Issue number	SEP
DOIs	https://doi.org/10.3389/fimmu.2018.02044
State	Published - Sep 13 2018

Keywords

2-photon imaging
CCL3/MIP1-alpha
Follicular helper T cells
Follicular regulatory T cells (Tfr)
Germinal centers

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10.3389/fimmu.2018.02044

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@article{e10bd239270e49478812ba064012de56,

title = "CCL3 promotes germinal center B cells sampling by follicular regulatory T cells in murine lymph nodes",

abstract = "Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.",

keywords = "2-photon imaging, CCL3/MIP1-alpha, Follicular helper T cells, Follicular regulatory T cells (Tfr), Germinal centers",

author = "Benet, \{Zachary L.\} and Matangi Marthi and Fang Ke and Rita Wu and Turner, \{Jackson S.\} and Gabayre, \{Jahan B.\} and Ivanitskiy, \{Michael I.\} and Sethi, \{Sahil S.\} and Grigorova, \{Irina L.\}",

note = "Funding Information: Supported by the American Heart Association (14PRE19960005) to ZB, by the Herman and Dorothy Miller Award for Innovative Immunology Research to JT and ZB, and National Institute of Health (R01 AI106806) to IG. The authors declare no competing financial interests. Publisher Copyright: {\textcopyright} 2018 Benet, Marthi, Ke, Wu, Turner, Gabayre, Ivanitskiy, Sethi and Grigorova.",

year = "2018",

month = sep,

day = "13",

doi = "10.3389/fimmu.2018.02044",

language = "English",

volume = "9",

journal = "Frontiers in immunology",

issn = "1664-3224",

number = "SEP",

}

TY - JOUR

T1 - CCL3 promotes germinal center B cells sampling by follicular regulatory T cells in murine lymph nodes

AU - Benet, Zachary L.

AU - Marthi, Matangi

AU - Ke, Fang

AU - Wu, Rita

AU - Turner, Jackson S.

AU - Gabayre, Jahan B.

AU - Ivanitskiy, Michael I.

AU - Sethi, Sahil S.

AU - Grigorova, Irina L.

N1 - Funding Information: Supported by the American Heart Association (14PRE19960005) to ZB, by the Herman and Dorothy Miller Award for Innovative Immunology Research to JT and ZB, and National Institute of Health (R01 AI106806) to IG. The authors declare no competing financial interests. Publisher Copyright: © 2018 Benet, Marthi, Ke, Wu, Turner, Gabayre, Ivanitskiy, Sethi and Grigorova.

PY - 2018/9/13

Y1 - 2018/9/13

N2 - Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.

AB - Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.

KW - 2-photon imaging

KW - CCL3/MIP1-alpha

KW - Follicular helper T cells

KW - Follicular regulatory T cells (Tfr)

KW - Germinal centers

UR - https://www.scopus.com/pages/publications/85054388836

U2 - 10.3389/fimmu.2018.02044

DO - 10.3389/fimmu.2018.02044

M3 - Article

C2 - 30271404

AN - SCOPUS:85054388836

SN - 1664-3224

VL - 9

JO - Frontiers in immunology

JF - Frontiers in immunology

IS - SEP

M1 - 2044

ER -

CCL3 promotes germinal center B cells sampling by follicular regulatory T cells in murine lymph nodes

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Keywords

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