Three-dimensional computer reconstruction of magnetic resonance images (MRI) of ten cadaveric specimens was used to determine carpal tunnel volume and carpal arch width. Magnetic resonance images-acquired data were compared with direct measurement of cadaveric carpal canal volume by means of a silicone-injection technique. Mean MRI three-dimensional reconstruction volume was 5.84 ml ± 1.24 ml. Mean silicone-mold volume was 4.73 ± 1.01 ml. A correction factor of 0.8161 was used to accurately calculate carpal tunnel volume from MRI-acquired data. There was no significant difference between calculated carpal tunnel volumes and silicone-mold volumes (p = 0.623), and there was a linear relationship between MRI-acquired volumes and silicone-mold volumes (correlation coefficient r = 0.97). Differences in MRI-volume determinations between observers were not significant (0.25 ≥ p>0.1). MRI three-dimensional reconstruction, a valid and reproducible technique for measuring carpal tunnel volume and dimensions, has considerable research potential for the evaluation of the relationship between the carpal canal and its contents before and after carpal tunnel release.