@article{09baa7af274741a59e07f692e2fda65a,
title = "Cargo crowding at actin-rich regions along axons causes local traffic jams",
abstract = "Steady axonal cargo flow is central to the functioning of healthy neurons. However, a substantial fraction of cargo in axons remains stationary up to several minutes. We examine the transport of precursors of synaptic vesicles (pre-SVs), endosomes and mitochondria in Caenorhabditis elegans touch receptor neurons, showing that stationary cargo are predominantly present at actin-rich regions along the neuronal process. Stationary vesicles at actin-rich regions increase the propensity of moving vesicles to stall at the same location, resulting in traffic jams arising from physical crowding. Such local traffic jams at actin-rich regions are likely to be a general feature of axonal transport since they also occur in Drosophila neurons. Repeated touch stimulation of C. elegans reduces the density of stationary pre-SVs, indicating that these traffic jams can act as both sources and sinks of vesicles. This suggests that vesicles trapped in actin-rich regions are functional reservoirs that may contribute to maintaining robust cargo flow in the neuron. A video abstract of this article can be found at: Video S1; Video S2.",
keywords = "Caenorhabditis elegans, Drosophila melanogaster, actin, axonal transport, neuronal stimulation, physical crowding, precursors of synaptic vesicles, reservoirs, stationary cargo, stationary vesicles, touch receptor neurons, traffic jams",
author = "Parul Sood and Kausalya Murthy and Vinod Kumar and Nonet, {Michael L.} and Menon, {Gautam I.} and Koushika, {Sandhya P.}",
note = "Funding Information: The authors gratefully acknowledge support from DAE Project 12-R&D-IMS-5.02-0202 to G.I.M. and S.P.K. and Howard Hughes Medical Institute (S.P.K.). We thank Dr Krishnamurthy facility in-charge CIFF-NCBS (Supported by SR/55/NM-36-2005), Sunaina Surana for LatA injections, Prof Krishanu Ray for Drosophila stocks and lab space, Aparna Ashok for acquiring movies of commissures of motor neurons in C. elegans. Some strains were provided by the CGC. We thank all Koushika lab members for comments on the manuscript. Salary support K.M.: DBT post-doctoral fellowship, V.K.: IMSc Prism DAE. Research costs: To S.P.K.: CSIR, HHMI-IECS. G.I.M.: DAE-SRC Fellowship and sabbatical support from the NUS, Singapore. Editorial Process File: The Editorial Process File is available in the online version of this article. Funding Information: The authors gratefully acknowledge support from DAE Project 12-R&D-IMS-5.02-0202 to G.I.M. and S.P.K. and Howard Hughes Medical Institute (S.P.K.). We thank Dr Krishnamurthy facility in-charge CIFF-NCBS (Supported by SR/55/NM-36-2005), Sunaina Surana for LatA injections, Prof Krishanu Ray for Drosophila stocks and lab space, Aparna Ashok for acquiring movies of commissures of motor neurons in C. elegans. Some strains were provided by the CGC. We thank all Koushika lab members for comments on the manuscript. Salary support K.M.: DBT post-doctoral fellowship, V.K.: IMSc Prism DAE. Research costs: To S.P.K.: CSIR, HHMI-IECS. G.I.M.: DAE-SRC Fellowship and sabbatical support from the NUS, Singapore. Publisher Copyright: {\textcopyright} 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",
year = "2018",
month = mar,
doi = "10.1111/tra.12544",
language = "English",
volume = "19",
pages = "166--181",
journal = "Traffic",
issn = "1398-9219",
number = "3",
}