TY - JOUR
T1 - Capturing Rare Conductance in Epithelia with Potentiometric-Scanning Ion Conductance Microscopy
AU - Zhou, Lushan
AU - Gong, Yongfeng
AU - Sunq, Abby
AU - Hou, Jianghui
AU - Baker, Lane A.
N1 - Funding Information:
We thank Washington University light microscopy and electron microscopy core for assistance. This work was supported by grants from NIDDK, Grant R01DK084059, the American Heart Association, Grant 0930050N, and the Department of Defense, Grant HDTRA1-11-16-BRCWMDBAA.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/10/4
Y1 - 2016/10/4
N2 - Tight junctions (TJs) are barrier forming structures of epithelia and can be described as tightly sealed intercellular spaces. Transport properties have been extensively studied for bicellular TJs (bTJs). Knowledge of the barrier functions of tricellular junctions (tTJs) are less well understood, due largely to a lack of proper techniques to locally measure discrete tTJ properties within a much larger area of epithelium. In this study, we use a nanoscale pipet to precisely locate tTJs within epithelia and measure the apparent local conductance of tTJs with a technique termed potentiometric scanning ion conductance microscopy (P-SICM). P-SICM shows the ability to differentiate transport through tTJs and bTJs, which was not possible with previous techniques and assays. We describe P-SICM investigations of both wild type and tricellulin overexpression Madin-Darby Canine Kidney (strain II, MDCKII) cells.
AB - Tight junctions (TJs) are barrier forming structures of epithelia and can be described as tightly sealed intercellular spaces. Transport properties have been extensively studied for bicellular TJs (bTJs). Knowledge of the barrier functions of tricellular junctions (tTJs) are less well understood, due largely to a lack of proper techniques to locally measure discrete tTJ properties within a much larger area of epithelium. In this study, we use a nanoscale pipet to precisely locate tTJs within epithelia and measure the apparent local conductance of tTJs with a technique termed potentiometric scanning ion conductance microscopy (P-SICM). P-SICM shows the ability to differentiate transport through tTJs and bTJs, which was not possible with previous techniques and assays. We describe P-SICM investigations of both wild type and tricellulin overexpression Madin-Darby Canine Kidney (strain II, MDCKII) cells.
UR - https://www.scopus.com/pages/publications/84989957285
U2 - 10.1021/acs.analchem.6b02392
DO - 10.1021/acs.analchem.6b02392
M3 - Article
C2 - 27618532
AN - SCOPUS:84989957285
SN - 0003-2700
VL - 88
SP - 9630
EP - 9637
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -