Capturing Rare Conductance in Epithelia with Potentiometric-Scanning Ion Conductance Microscopy

Lushan Zhou, Yongfeng Gong, Abby Sunq, Jianghui Hou, Lane A. Baker

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Tight junctions (TJs) are barrier forming structures of epithelia and can be described as tightly sealed intercellular spaces. Transport properties have been extensively studied for bicellular TJs (bTJs). Knowledge of the barrier functions of tricellular junctions (tTJs) are less well understood, due largely to a lack of proper techniques to locally measure discrete tTJ properties within a much larger area of epithelium. In this study, we use a nanoscale pipet to precisely locate tTJs within epithelia and measure the apparent local conductance of tTJs with a technique termed potentiometric scanning ion conductance microscopy (P-SICM). P-SICM shows the ability to differentiate transport through tTJs and bTJs, which was not possible with previous techniques and assays. We describe P-SICM investigations of both wild type and tricellulin overexpression Madin-Darby Canine Kidney (strain II, MDCKII) cells.

Original languageEnglish
Pages (from-to)9630-9637
Number of pages8
JournalAnalytical Chemistry
Volume88
Issue number19
DOIs
StatePublished - Oct 4 2016

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