TY - JOUR
T1 - Captopril reduces the severity of bowel damage in a neonatal rat model of necrotizing enterocolitis
AU - Zani, Augusto
AU - Eaton, Simon
AU - Leon, Francesco Fascetti
AU - Malerba, Alberto
AU - Hall, Nigel J.
AU - De Coppi, Paolo
AU - Smith, Virpi V.
AU - Pierro, Agostino
PY - 2008/2
Y1 - 2008/2
N2 - Background/Purpose: Selective mesenteric ischemia may result from activation of the renin-angiotensin system during periods of shock and is implicated in the pathogenesis of neonatal necrotizing enterocolitis (NEC). We investigated the effectiveness of captopril, an angiotensin-converting enzyme inhibitor, in reducing the severity of bowel damage in a neonatal rat model of NEC. Methods: Necrotizing enterocolitis was induced by a combination of gavage feeding of hypertonic formula, hypoxia, and oral lipopolysaccharide (LPS). Rats were randomly divided into 3 groups: group A, control (breast fed; n = 20); group B, NEC (gavage/hypoxia/LPS; n = 31); group C, NEC with captopril 20 mg/kg per dose with the formula for 4 days (gavage/hypoxia/LPS/captopril; n = 35). Pups were killed after 4 days. Incidence of NEC was evaluated microscopically. Results: Severity of bowel damage was higher in the NEC group compared to controls and was reduced by administration of captopril. Dilatation of the intestinal vasculature was observed in the captopril group. There were no cases of NEC in the controls; the incidence increased to 55% in NEC group and reduced to 29% by captopril. Conclusions: In this model of neonatal NEC, captopril supplementation of formula reduces the severity of intestinal damage and the incidence of NEC, presumably by affecting mesenteric blood flow.
AB - Background/Purpose: Selective mesenteric ischemia may result from activation of the renin-angiotensin system during periods of shock and is implicated in the pathogenesis of neonatal necrotizing enterocolitis (NEC). We investigated the effectiveness of captopril, an angiotensin-converting enzyme inhibitor, in reducing the severity of bowel damage in a neonatal rat model of NEC. Methods: Necrotizing enterocolitis was induced by a combination of gavage feeding of hypertonic formula, hypoxia, and oral lipopolysaccharide (LPS). Rats were randomly divided into 3 groups: group A, control (breast fed; n = 20); group B, NEC (gavage/hypoxia/LPS; n = 31); group C, NEC with captopril 20 mg/kg per dose with the formula for 4 days (gavage/hypoxia/LPS/captopril; n = 35). Pups were killed after 4 days. Incidence of NEC was evaluated microscopically. Results: Severity of bowel damage was higher in the NEC group compared to controls and was reduced by administration of captopril. Dilatation of the intestinal vasculature was observed in the captopril group. There were no cases of NEC in the controls; the incidence increased to 55% in NEC group and reduced to 29% by captopril. Conclusions: In this model of neonatal NEC, captopril supplementation of formula reduces the severity of intestinal damage and the incidence of NEC, presumably by affecting mesenteric blood flow.
KW - Angiotensin-converting enzyme inhibitor
KW - Captopril
KW - Necrotizing enterocolitis
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=38949087916&partnerID=8YFLogxK
U2 - 10.1016/j.jpedsurg.2007.10.022
DO - 10.1016/j.jpedsurg.2007.10.022
M3 - Article
C2 - 18280280
AN - SCOPUS:38949087916
SN - 0022-3468
VL - 43
SP - 308
EP - 314
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
IS - 2
ER -