Capping protein levels influence actin assembly and cell motility in dictyostelium

Christopher Hug, Patrick Y. Jay, Indira Reddy, James G. McNally, Paul C. Bridgman, Elliot L. Elson, John A. Cooper

Research output: Contribution to journalArticlepeer-review

148 Scopus citations


Actin assembly is important for cell motility, but the mechanism of assembly and how it relates to motility in vivo is largely unknown. In vitro, actin assembly can be controlled by proteins, such as capping protein, that bind filament ends. To investigate the function of actin assembly in vivo, we altered the levels of capping protein in Dictyostelium cells and found changes in resting and chemoattractant-induced actin assembly that were consistent with the in vitro properties of capping protein in capping but not nucleation. Significantly, overexpressers moved faster and underexpressers moved slower than control cells. Mutants also exhibited changes in cytoskeleton architecture. These results provide insights into in vivo actin assembly and the role of the actin cytoskeleton in motility.

Original languageEnglish
Pages (from-to)591-600
Number of pages10
Issue number4
StatePublished - May 19 1995


Dive into the research topics of 'Capping protein levels influence actin assembly and cell motility in dictyostelium'. Together they form a unique fingerprint.

Cite this