TY - JOUR
T1 - Cancer Stem Cell Signaling during Repopulation in Head and Neck Cancer
AU - Wilson, George D.
AU - Thibodeau, Bryan J.
AU - Fortier, Laura E.
AU - Pruetz, Barbara L.
AU - Galoforo, Sandra
AU - Marples, Brian
AU - Baschnagel, Andrew M.
AU - Akervall, Jan
AU - Huang, Jiayi
N1 - Publisher Copyright:
© 2016 George D. Wilson et al.
PY - 2016
Y1 - 2016
N2 - The aim of the study was to investigate cancer stem signaling during the repopulation response of a head and neck squamous cell cancer (HNSCC) xenograft after radiation treatment. Xenografts were generated from low passage HNSCC cells and were treated with either sham radiation or 15 Gy in one fraction. At different time points, days 0, 3, and 10 for controls and days 4, 7, 12, and 21, after irradiation, 3 tumors per group were harvested for global gene expression, pathway analysis, and immunohistochemical evaluation. 316 genes were identified that were associated with a series of stem cell-related genes and were differentially expressed (p ≤ 0.01 and 1.5-fold) at a minimum of one time point in UT-SCC-14 xenografts after radiation. The largest network of genes that showed significant changes after irradiation was associated with CD44, NOTCH1, and MET. c-MET and ALDH1A3 staining correlated with the changes in gene expression. A clear pattern emerged that was consistent with the growth inhibition data in that genes associated with stem cell pathways were most active at day 7 and day 12 after irradiation. The MET/CD44 axis seemed to be an important component of the repopulation response.
AB - The aim of the study was to investigate cancer stem signaling during the repopulation response of a head and neck squamous cell cancer (HNSCC) xenograft after radiation treatment. Xenografts were generated from low passage HNSCC cells and were treated with either sham radiation or 15 Gy in one fraction. At different time points, days 0, 3, and 10 for controls and days 4, 7, 12, and 21, after irradiation, 3 tumors per group were harvested for global gene expression, pathway analysis, and immunohistochemical evaluation. 316 genes were identified that were associated with a series of stem cell-related genes and were differentially expressed (p ≤ 0.01 and 1.5-fold) at a minimum of one time point in UT-SCC-14 xenografts after radiation. The largest network of genes that showed significant changes after irradiation was associated with CD44, NOTCH1, and MET. c-MET and ALDH1A3 staining correlated with the changes in gene expression. A clear pattern emerged that was consistent with the growth inhibition data in that genes associated with stem cell pathways were most active at day 7 and day 12 after irradiation. The MET/CD44 axis seemed to be an important component of the repopulation response.
UR - http://www.scopus.com/inward/record.url?scp=84961384078&partnerID=8YFLogxK
U2 - 10.1155/2016/1894782
DO - 10.1155/2016/1894782
M3 - Article
C2 - 26880935
AN - SCOPUS:84961384078
SN - 1687-9678
VL - 2016
JO - Stem Cells International
JF - Stem Cells International
M1 - 1894782
ER -