CAMP factor is not essential for systemic virulence of Group B Streptococcus

Mary E. Hensler, Darin Quach, Chia Jun Hsieh, Kelly S. Doran, Victor Nizet

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37 Scopus citations


The Gram-positive pathogen Group B Streptococcus (GBS) is the leading cause of bacterial pneumonia, sepsis, and meningitis in human newborns. GBS elaborates a pore-forming toxin known as CAMP factor that synergizes with Staphylococcus aureus β-toxin, generating a co-hemolytic reaction useful in identification of GBS in the clinical laboratory. To evaluate the indirect evidence implicating CAMP factor in GBS pathogenesis, the cfb gene encoding the pore-forming cytotoxin was deleted by precise allelic replacement. The virulence properties of the CAMP factor mutant were then explored by a series of in vitro and in vivo assays. Compared to wild-type, the isogenic GBS Δcfb mutant demonstrated equivalent phagocyte resistance and endothelial cell invasiveness and also retained full virulence in a mouse model of infection. Our data suggest that CAMP factor expressed in its native context is not essential for systemic virulence of GBS.

Original languageEnglish
Pages (from-to)84-88
Number of pages5
JournalMicrobial Pathogenesis
Issue number1
StatePublished - Jan 2008


  • Bacterial infection
  • Hemolysin
  • Pore-forming toxin
  • Streptococcus agalactiae


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