Calmodulin regulates synaptic plasticity in the anterior cingulate cortex and behavioral responses: A microelectroporation study in adult rodents

Feng Wei, Xia Ming Xia, Jianrong Tang, Hushan Ao, Shanelle Ko, Jason Liauw, Chang Shen Qiu, Min Zhuo

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

We developed a microelectroporation method for the transfer of genes into neurons in the cerebral cortex of adult rodents, both rats and mice. We selectively expressed either green-fluorescent protein (GFP) or a Ca 2+ -binding deficient calmodulin (CaM) mutant in the anterior cingulate cortex (ACC). In mice that expressed GFP, positive neuronal cell bodies were found specifically at the injection site in the ACC. Mice that expressed CaM12, a mutant CaM with two impaired Ca2+ binding sites in the N-terminal lobe, exhibited significant changes in vocalization, locomotion, and sensory functions. Long-term potentiation and long-term depression, two major forms of central plasticity, were completely abolished by expression of CaM12. Mice that expressed CaM 34, a mutant CaM with two impaired Ca 2+ binding sites in the C-terminal lobe, did not show any significant behavioral or electrophysiological alterations. These findings provide strong evidence that CaM is critical for bidirectional synaptic plasticity. This new method will be useful for investigating gene function in specific brain regions of freely moving animals. Furthermore, this approach also may facilitate gene therapy in adult human brains.

Original languageEnglish
Pages (from-to)8402-8409
Number of pages8
JournalJournal of Neuroscience
Volume23
Issue number23
DOIs
StatePublished - Sep 10 2003

Keywords

  • Anterior cingulate cortex
  • Calmodulin
  • Electroporation
  • Gene transfer
  • Mice
  • Synaptic plasticity

Fingerprint

Dive into the research topics of 'Calmodulin regulates synaptic plasticity in the anterior cingulate cortex and behavioral responses: A microelectroporation study in adult rodents'. Together they form a unique fingerprint.

Cite this