TY - JOUR
T1 - Calcium imaging in populations of olfactory neurons by planar illumination microscopy
AU - Holy, Timothy E.
PY - 2014/3
Y1 - 2014/3
N2 - Neurons in the olfactory system display extraordinary functional diversity, which at the level of sensory neurons arises from the expression of one out of several hundred distinct receptor types. To cope with this diversity, one approach is to use techniques that can record sensory responses from many neurons simultaneously. We have developed a form of light-sheet microscopy, called objective-coupled planar illumination (OCPI) microscopy, that is well suited to recording at high signal-to-noise ratios from large neuronal populations. Because OCPI microscopy illuminates the entire field simultaneously, it allows fast imaging without compromising field of view. At current camera speeds, pixels can be acquired more than 100-fold faster than by point-scanning fluorescence microscopy. Here we describe the theory, advantages, and practical implementation of planar illumination and briefly discuss its application to neuronal recording in the mouse vomeronasal organ. We also provide a brief protocol, in which a mouse is pretreated with dye for 1 wk to allow labeling of the sensory neurons before stimulation and imaging.
AB - Neurons in the olfactory system display extraordinary functional diversity, which at the level of sensory neurons arises from the expression of one out of several hundred distinct receptor types. To cope with this diversity, one approach is to use techniques that can record sensory responses from many neurons simultaneously. We have developed a form of light-sheet microscopy, called objective-coupled planar illumination (OCPI) microscopy, that is well suited to recording at high signal-to-noise ratios from large neuronal populations. Because OCPI microscopy illuminates the entire field simultaneously, it allows fast imaging without compromising field of view. At current camera speeds, pixels can be acquired more than 100-fold faster than by point-scanning fluorescence microscopy. Here we describe the theory, advantages, and practical implementation of planar illumination and briefly discuss its application to neuronal recording in the mouse vomeronasal organ. We also provide a brief protocol, in which a mouse is pretreated with dye for 1 wk to allow labeling of the sensory neurons before stimulation and imaging.
UR - http://www.scopus.com/inward/record.url?scp=84895447084&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot081174
DO - 10.1101/pdb.prot081174
M3 - Article
C2 - 24591697
AN - SCOPUS:84895447084
VL - 2014
SP - 317
EP - 323
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
IS - 3
ER -