TY - JOUR
T1 - "Caged" phenylephrine
T2 - Development and application to probe the mechanism of α-receptor-mediated vasoconstriction
AU - Muralidharan, S.
AU - Maher, G. M.
AU - Boyle, W. A.
AU - Nerbonne, J. M.
PY - 1993/6/1
Y1 - 1993/6/1
N2 - A "caged" analogue of the α-adrenergic receptor agonist phenylephrine (PE) was prepared by exploiting the 2-nitrobenzyl protecting group and using a synthetic procedure developed to permit preferential derivatization at the amino group. On isolated adult rat mesenteric arterioles, caged-PE had no measurable effects at concentrations up to 100 μM; 0.5-ms light flashes in the presence of caged-PE, however, produced marked and dose-dependent vasoconstriction. Flash-induced vasoconstrictions were blocked by the α-receptor antagonist phentolamine and were unaffected by the β-receptor antagonist propranolol, indicating that the light-induced responses reflect the selective activation of α-adrenergic receptors. After a single flash, a large transient decrease in vessel diameter was recorded, and in most vessels, this was followed by a smaller, sustained constriction. The sustained component of the contraction was selectively eliminated when Ca2+ was removed from the bath, which suggests that different mechanisms underlie the transient and the sustained responses to PE. The responses to single flashes of varying intensities occurred with a mean latency of 460 ms, which is consistent with the intermediacy of several steps between α-receptor activation and contraction. We anticipate that it will be possible to extend this approach to develop caged analogues of other neurotransmitters for mechanistic and kinetic studies.
AB - A "caged" analogue of the α-adrenergic receptor agonist phenylephrine (PE) was prepared by exploiting the 2-nitrobenzyl protecting group and using a synthetic procedure developed to permit preferential derivatization at the amino group. On isolated adult rat mesenteric arterioles, caged-PE had no measurable effects at concentrations up to 100 μM; 0.5-ms light flashes in the presence of caged-PE, however, produced marked and dose-dependent vasoconstriction. Flash-induced vasoconstrictions were blocked by the α-receptor antagonist phentolamine and were unaffected by the β-receptor antagonist propranolol, indicating that the light-induced responses reflect the selective activation of α-adrenergic receptors. After a single flash, a large transient decrease in vessel diameter was recorded, and in most vessels, this was followed by a smaller, sustained constriction. The sustained component of the contraction was selectively eliminated when Ca2+ was removed from the bath, which suggests that different mechanisms underlie the transient and the sustained responses to PE. The responses to single flashes of varying intensities occurred with a mean latency of 460 ms, which is consistent with the intermediacy of several steps between α-receptor activation and contraction. We anticipate that it will be possible to extend this approach to develop caged analogues of other neurotransmitters for mechanistic and kinetic studies.
KW - Calcium
KW - Vascular smooth muscle
KW - α-adrenergic receptor
UR - http://www.scopus.com/inward/record.url?scp=0027200349&partnerID=8YFLogxK
M3 - Article
C2 - 8389474
AN - SCOPUS:0027200349
SN - 0027-8424
VL - 90
SP - 5199
EP - 5203
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -