Caffeine combined with sedative/anesthetic drugs triggers widespread neuroapoptosis in a mouse model of prematurity

Omar Hoseá Cabrera, Shawn David O’Connor, Brant Stephen Swiney, Patricia Salinas-Contreras, Francesca Maria Manzella, George Townsend Taylor, Kevin Kiyoshi Noguchi

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Objectives: Caffeine (CAF) and sedative/anesthetic drugs (SADs) are often coadministered to premature infants in the neonatal intensive care unit (NICU). While SAD neurotoxicity in the developing brain is well established, it is not fully clear whether CAF interacts with SADs and whether this interaction is detrimental. Using a mouse model of prematurity, we hypothesized that CAF would increase apoptotic neurotoxicity when coadministered with SADs. Methods: Postnatal day 3 mice were treated with vehicle or 80 mg/kg CAF prior to challenge with 6 mg/kg midazolam, 40 mg/kg ketamine, or 40 lg/kg fentanyl. Six hours later, pups were sacrificed for activated caspase 3 (AC3) immunohistochemistry, and number of AC3 positive cells per mm3 throughout neocortex, hippocampus, caudate, thalamus, and colliculi was analyzed. Results: CAF caused a statistically significant increase in AC3 positive cells when coadministered with midazolam (p = 0.002), ketamine (p = 0.014), or fentanyl (p < 0.001). Our composite dataset suggests that the addition of CAF to these SADs has a supra-additive effect, causing more neurotoxicity than expected. Conclusions: CAF may augment the neurotoxic action of SADs indicated for neonatal sedation/ anesthesia in the NICU by triggering widespread apoptosis in the developing brains of premature infants.

Original languageEnglish
Pages (from-to)2734-2741
Number of pages8
JournalJournal of Maternal-Fetal and Neonatal Medicine
Issue number22
StatePublished - 2017


  • Apoptosis
  • Caffeine
  • Fentanyl
  • Ketamine
  • Midazolam
  • Premature infant

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