TY - JOUR
T1 - Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing
AU - Jantsch, Verena
AU - Tang, Lois
AU - Pasierbek, Pawel
AU - Penkner, Alexandra
AU - Nayak, Sudhir
AU - Baudrimont, Antoine
AU - Schedl, Tim
AU - Gartner, Anton
AU - Loidl, Josef
PY - 2007/12
Y1 - 2007/12
N2 - A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in increased embryonic lethality and a Him phenotype. Furthermore, retarded and asynchronous nuclear reorganization as well as reduced homologous synapsis occur during meiotic prophase. Accumulation of recombination protein RAD-51 in meiotic nuclei suggests disturbed repair of double-stranded DNA breaks. Nuclei in prom-1 mutant gonads timely complete mitotic proliferation and premeiotic replication, but they undergo prolonged delay upon meiotic entry. We, therefore, propose that prom-1 regulates the timely progression through meiotic prophase I and that in its absence the recognition of homologous chromosomes is strongly impaired.
AB - A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in increased embryonic lethality and a Him phenotype. Furthermore, retarded and asynchronous nuclear reorganization as well as reduced homologous synapsis occur during meiotic prophase. Accumulation of recombination protein RAD-51 in meiotic nuclei suggests disturbed repair of double-stranded DNA breaks. Nuclei in prom-1 mutant gonads timely complete mitotic proliferation and premeiotic replication, but they undergo prolonged delay upon meiotic entry. We, therefore, propose that prom-1 regulates the timely progression through meiotic prophase I and that in its absence the recognition of homologous chromosomes is strongly impaired.
UR - http://www.scopus.com/inward/record.url?scp=37049023879&partnerID=8YFLogxK
U2 - 10.1091/mbc.E07-03-0243
DO - 10.1091/mbc.E07-03-0243
M3 - Article
C2 - 17914060
AN - SCOPUS:37049023879
SN - 1059-1524
VL - 18
SP - 4911
EP - 4920
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 12
ER -