BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin

Sonam Verma, Amandeep Kaur Kang, Rahul Pal, Satish Kumar Gupta

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The mechanism by which interferon-gamma (IFN-γ) downregulates trophoblast invasion needs further investigation. Treatment of HTR-8/SVneo cells with IFN-γ led to a decrease in their invasion concomitant with an increased expression of BST2. Silencing of BST2 by siRNA showed a significant increase in their invasion and spreading after treatment with IFN-γ as well as downregulated expression of E-cadherin. Further, STAT1 silencing inhibited the IFN-γ-dependent increase in the expression of BST2 and E-cadherin. Treatment of HTR-8/SVneo cells with IFN-γ led to the activation of AKT, and its inhibition with PI3K inhibitor abrogated IFN-γ-mediated decrease in invasion/spreading and downregulated BST2 and E-cadherin expression. Collectively, IFN-γ decreases the invasion of HTR-8/SVneo cells by STAT1 and AKT activation via increased expression of BST2 and E-cadherin.

Original languageEnglish
Pages (from-to)24-41
Number of pages18
JournalCell Adhesion and Migration
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2020

Keywords

  • AKT
  • BST2
  • E-cadherin
  • IFN-γ
  • STAT1
  • trophoblast invasion

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