@article{d259dc96718048709916514a90d1a9aa,
title = "BRUTUS and its paralogs, BTS LIKE1 and BTS LIKE2, encode important negative regulators of the iron deficiency response in Arabidopsis thaliana",
abstract = "Iron (Fe) is required for plant health, but it can also be toxic when present in excess. Therefore, Fe levels must be tightly controlled. The Arabidopsis thaliana E3 ligase BRUTUS (BTS) is involved in the negative regulation of the Fe deficiency response and we show here that the two A. thaliana BTS paralogs, BTS LIKE1 (BTSL1) and BTS LIKE2 (BTSL2) encode proteins that act redundantly as negative regulators of the Fe deficiency response. Loss of both of these E3 ligases enhances tolerance to Fe deficiency. We further generated a triple mutant with loss of both BTS paralogs and a partial loss of BTS expression that exhibits even greater tolerance to Fe-deficient conditions and increased Fe accumulation without any resulting Fe toxicity effects. Finally, we identified a mutant carrying a novel missense mutation of BTS that exhibits an Fe deficiency response in the root when grown under both Fe-deficient and Fe-sufficient conditions, leading to Fe toxicity when plants are grown under Fe-sufficient conditions.",
author = "Hindt, {Maria N.} and Akmakjian, {Garo Z.} and Pivarski, {Kara L.} and Tracy Punshon and Ivan Baxter and Salt, {David E.} and Guerinot, {Mary Lou}",
note = "Funding Information: Microarray analysis was carried out at the Geisel School of Medicine in the Genomics Shared Resource, which was established by equipment grants from the NIH and NSF and is supported in part by a Cancer Center Core Grant (P30CA023108) from the National Cancer Institute. We thank Amanda Socha for help with RNA extraction for microarray analysis and Carol Ringelberg for her help with microarray data analysis. We thank Suzana Car for her help with SXRF experiments at NSLS. We thank Sue Wirick and William Rao for their help at X26A and Ryan Tappero for his help at X27A. X26A is supported by the Department of Energy (DOE) - Geosciences (DE-FG02-92ER14244 to The University of Chicago - CARS). Use of the NSLS was supported by DOE under Contract No. DE-AC02-98CH10886. X27A is supported in part by the U.S. Department of Energy - Geosciences (DE-FG02-92ER14244 to The University of Chicago - CARS) and Brookhaven National Laboratory-Department of Environmental Sciences. Use of the NSLS was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-98CH10886. We thank Sam Webb and Benjamin Kocar for their help at Beamline 2-3 at SSRL. Publisher Copyright: {\textcopyright} 2017 The Royal Society of Chemistry.",
year = "2017",
month = jul,
doi = "10.1039/c7mt00152e",
language = "English",
volume = "9",
pages = "876--890",
journal = "Metallomics",
issn = "1756-5901",
number = "7",
}