Brugia malayi: Effects of nitazoxanide and tizoxanide on adult worms and microfilariae of filarial nematodes

Ramakrishna U. Rao, Yuefang Huang, Kerstin Fischer, Peter U. Fischer, Gary J. Weil

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

There is an urgent need for safe and effective antifilarials. Prior studies have shown that the nitazoxanide (NTZ) exhibits broad activity against anaerobic bacteria, protozoa, and certain intestinal helminths. We examined the effects of NTZ and tizoxanide (TZ) on Brugia malayi nematodes in vitro and in vivo. In vitro, NTZ and TZ reduced worm motility and viability in a dose-dependent manner. Worm viability was reduced by 50% with both compounds at 2.5 and 20 μg/ml killed adult worms. NTZ or TZ (5 μg/ml) significantly reduced microfilaria release. These compounds blocked worm's embryogenesis, and decreased microfilarial motility and viability. Treated worms had damaged cuticles and abnormal mitochondria. Wolbachia were not cleared by NTZ or TZ treatment. Neither NTZ nor TZ cleared adult worms or microfilariae in infected gerbils. These results show that NTZ and TZ have potent effects on B. malayi nematodes in vitro. However, they were not effective in vivo.

Original languageEnglish
Pages (from-to)38-45
Number of pages8
JournalExperimental Parasitology
Volume121
Issue number1
DOIs
StatePublished - Jan 2009

Keywords

  • Albendazole (Alb)
  • Brugia malayi
  • Diethylcarbamazine (DEC)
  • Filariasis
  • Microfilariae (MF)
  • Nitazoxanide (NTZ)
  • Real-time quantitative PCR (qPCR)
  • Tizoxanide (TZ)
  • Wolbachia

Fingerprint

Dive into the research topics of 'Brugia malayi: Effects of nitazoxanide and tizoxanide on adult worms and microfilariae of filarial nematodes'. Together they form a unique fingerprint.

Cite this