Bromination from the macroscopic level to the tracer radiochemical level: 76Br radiolabeling of aromatic compounds via electrophilic substitution

Dong Zhou, Haibing Zhou, Carl C. Jenks, Jason S. Lewis, John A. Katzenellenbogen, Michael J. Welch

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

No-carrier-added (NCA) 76Br labeling of 4-(5-acetoxy-7- bromobenzoxazol-2-yl)phenyl acetate, a diacetate-protected estrogen-receptor beta (ERβ) selective ligand, was carried out successfully using [ 76Br]bromide ion. The labeling was achieved via oxidative electrophilic destannylation of an organotin precursor molecule by modification of the leaving group (from Bu 3Sn to Me 3Sn) and the addition of methanol to the reaction mixture. The differences between the oxidative bromination reaction under small-scale macroscopic vs tracer level radiochemical conditions were explored in terms of effective brominating agents, which depend greatly on the nature of the solvent during the radiochemical bromination, and the potential interference by trace levels of highly reactive impurities in the reaction that compete for the desired bromination at the NCA level. Our observations, and our development of experimental protocols for successful radiobromination at the tracer NCA-scale, should be applicable to the synthesis of other radiobromine-labeled organic compounds of potential interest as PET radiopharmaceuticals and radiotherapy agents.

Original languageEnglish
Pages (from-to)808-816
Number of pages9
JournalBioconjugate Chemistry
Volume20
Issue number4
DOIs
StatePublished - Apr 15 2009

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