Bright fluorescent nanoparticles for developing potential optical imaging contrast agents

Guorong Sun, Mikhail Y. Berezin, Jinda Fan, Hyeran Lee, Jun Ma, Ke Zhang, Karen L. Wooley, Samuel Achilefu

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Fluorescent cross-linked nanoparticles with variable fluorophore loading amounts, locations, and particle sizes were synthesized from sequential one-pot functionalization/cross-linking of block copolymer micelles with amine-terminated dye and cross-linker molecules, via reductive amination and amidation. The fluorescence quantum yield and brightness of these nanoparticles were evaluated by steady-state and dynamic fluorescence methods. The results demonstrate that the quantum yield and brightness of the fluorescent nanoparticles correlated directly with the number of dyes/nanoparticle and the nanoparticle size. A strategy to increase the fluorescence brightness of nanoparticles with fluorescein and near-infrared dyes is proposed.

Original languageEnglish
Pages (from-to)548-558
Number of pages11
JournalNanoscale
Volume2
Issue number4
DOIs
StatePublished - 2010

Fingerprint

Dive into the research topics of 'Bright fluorescent nanoparticles for developing potential optical imaging contrast agents'. Together they form a unique fingerprint.

Cite this