@inbook{1b926759db6c443db1c636e06452c7b9,
title = "Brief introduction to fluorescence correlation spectroscopy",
abstract = "Fluorescence correlation spectroscopy (FCS) measures rates of transport (diffusion coefficients, convection velocities) and chemical reactions (rate constants) in equilibrium or nonequilibrium steady-state systems without the need for a perturbation of the state of the system. The rates are extracted from a record of fluorescence fluctuations observed from an open laser-illuminated observation volume. In addition, FCS provides the number of measured fluorescent molecules in the observation volume and, therefore, their molecular brightness. This enables sensitive measurements of aggregation of the fluorescent system components. FCS is now used as a routine tool for studying systems in physics, chemistry, and biology. Recent extensions of the fluorescence fluctuation approach have included methods based on imaging and cross correlation. Because of the sensitivity and chemical specificity of fluorescence detection and also the ability to form a very small diffraction-limited detection volume, FCS is especially useful for studies of dynamic molecular processes in living biological cells. FCS provides a window on mesoscopic systems and is closely related to fluorescence methods for studying single molecules.",
keywords = "Chemical Kinetics, Correlation Function, Diffusion, Fluctuation, Fluorescence",
author = "Elson, {Elliot L.}",
note = "Funding Information: The development and application of FCS at Cornell and beyond resulted from the work of many people. I can list only a few whose contributions seem to me notably conspicuous. All of the work at Cornell could not have flourished without the wisdom, enthusiasm, and experimental expertise of Watt Webb. The initial demonstration of FCS was entirely dependent on the persistence, skill, and technical know-how of Douglas Magde. Dennis Koppel and Daniel Axelrod established the microscope-based versions of FCS and developed our version of FPR. Joseph Schlessinger was the prime mover in the early applications of these methods to cells. In later work, Hong Qian provided a number of important contributions including, among others, analyses of the FCS optical system, measurement noise, and the high moment approach to measuring molecule number and brightness. Saveez Saffarian extended the analysis of measurement noise and carried out studies of the aggregation of EGF receptors and of a matrix metalloproteinase Brownian ratchet. I would like to thank Michael Edidin for his advice and Ken Jacobson for his early participation in our measurements on celIs. I am also grateful to Rudolf Rigler for the work that he and his colleagues have done that led to the establishment of FCS as a widely accessible measurement method as well as for the many interesting and wide ranging applications that they have provided. Finally, I thank NIH (R01-GM084200) and NSF (CMMI 0826518) for financial support.",
year = "2013",
doi = "10.1016/B978-0-12-388422-0.00002-9",
language = "English",
isbn = "9780123884220",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "11--41",
booktitle = "Fluorescence Fluctuation Spectroscopy (FFS), Part A",
}