Breast cancer detection by phased array localization of fluorescent molecular beacons

Britton Chance, Y. Chen, X. Intes, J. Zhang, J. Glickson, D. Blessington, L. Zhou, G. Zheng, P. Lu, S. Achilefu, C. Tung

Research output: Contribution to journalConference articlepeer-review

Abstract

Recent developments in molecular beacons offer a way to selectively tag various pre-cancer/cancer signatures and provide high tumor to background contrast. Near-infrared (NIR) imaging can probe tissue deeply up to a couple of centimeters; thus it possesses the potential for non-invasively detection of breast or lymph node cancer. To increase the sensitivity in detecting fluorescent photons and the accuracy of tumor localization, phase cancellation (in- and anti-phase) device is employed. The source-detector pair scans the tissue surface in multiple directions and the localization image can be obtained using goniometric reconstruction. Tumor bearing mouse model with injection of fluorescent contrast agents is used to simulate the human breast tumor labeled with molecular beacons, and has been accurately localized with a depth up to 3 cm. The delivery of the molecular beacon is verified by the cryo-imaging with 3-D low temperature fluorescence scanner. The phase cancellation instrument has the potential for in vivo tumor diagnosis and imaging, and the accuracy of the localization suggests that this system could help to guide the clinical fine-needle biopsy. Also a handheld self-examination device using phased cancellation principle would be complementary to X-ray mammography and provide add-on information on early diagnosis and localization of breast tumor.

Original languageEnglish
Pages (from-to)493-509
Number of pages17
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5068
StatePublished - 2002
EventSaratov Fall Meeting 2002 Optical Technologies in Biophysics and Medicine IV - Saratov, Russian Federation
Duration: Oct 1 2002Oct 4 2002

Keywords

  • Breast cancer
  • Fluorescence
  • Molecular beacon
  • NIR imaging
  • Phased array

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