Brca1 mutations in the coiled-coil domain impede Rad51 loading on DNA and mouse development

J. J. Krais; N. Johnson

doi:10.1080/23723556.2020.1786345

Brca1 mutations in the coiled-coil domain impede Rad51 loading on DNA and mouse development

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Abstract

We recently developed a Brca1 coiled-coil mutant mouse model (Brca1^CC). Brca1^CC/CC results in embryonic lethality, with a fraction of mice reaching birth but with defects that parallel Fanconi anemia. Brca1^CC/CC cells lacked Rad51 foci and were PARP inhibitor sensitive. Strikingly, inter-crossing with Brca1^Δ11 generated Brca1 ^CC/Δ11 mice that were developmentally normal.

Original language	English
Article number	1786345
Journal	Molecular and Cellular Oncology
DOIs	https://doi.org/10.1080/23723556.2020.1786345
State	Published - 2020

Keywords

BRCA1
DNA end resection
Fanconi anemia
PALB2
RAD51 loading
coiled-coil
homologous recombination

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10.1080/23723556.2020.1786345

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title = " Brca1 mutations in the coiled-coil domain impede Rad51 loading on DNA and mouse development",

abstract = "We recently developed a Brca1 coiled-coil mutant mouse model (Brca1CC). Brca1CC/CC results in embryonic lethality, with a fraction of mice reaching birth but with defects that parallel Fanconi anemia. Brca1CC/CC cells lacked Rad51 foci and were PARP inhibitor sensitive. Strikingly, inter-crossing with Brca1Δ11 generated Brca1 CC/Δ11 mice that were developmentally normal.",

keywords = "BRCA1, DNA end resection, Fanconi anemia, PALB2, RAD51 loading, coiled-coil, homologous recombination",

author = "Krais, {J. J.} and N. Johnson",

note = "Publisher Copyright: {\textcopyright} 2020, {\textcopyright} 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.",

year = "2020",

doi = "10.1080/23723556.2020.1786345",

language = "English",

journal = "Molecular and Cellular Oncology",

issn = "2372-3556",

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T1 - Brca1 mutations in the coiled-coil domain impede Rad51 loading on DNA and mouse development

AU - Krais, J. J.

AU - Johnson, N.

PY - 2020

Y1 - 2020

N2 - We recently developed a Brca1 coiled-coil mutant mouse model (Brca1CC). Brca1CC/CC results in embryonic lethality, with a fraction of mice reaching birth but with defects that parallel Fanconi anemia. Brca1CC/CC cells lacked Rad51 foci and were PARP inhibitor sensitive. Strikingly, inter-crossing with Brca1Δ11 generated Brca1 CC/Δ11 mice that were developmentally normal.

AB - We recently developed a Brca1 coiled-coil mutant mouse model (Brca1CC). Brca1CC/CC results in embryonic lethality, with a fraction of mice reaching birth but with defects that parallel Fanconi anemia. Brca1CC/CC cells lacked Rad51 foci and were PARP inhibitor sensitive. Strikingly, inter-crossing with Brca1Δ11 generated Brca1 CC/Δ11 mice that were developmentally normal.

KW - BRCA1

KW - DNA end resection

KW - Fanconi anemia

KW - PALB2

KW - RAD51 loading

KW - coiled-coil

KW - homologous recombination

UR - http://www.scopus.com/inward/record.url?scp=85088264014&partnerID=8YFLogxK

U2 - 10.1080/23723556.2020.1786345

DO - 10.1080/23723556.2020.1786345

M3 - Article

AN - SCOPUS:85088264014

SN - 2372-3556

JO - Molecular and Cellular Oncology

JF - Molecular and Cellular Oncology

M1 - 1786345

ER -

Brca1 mutations in the coiled-coil domain impede Rad51 loading on DNA and mouse development

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Keywords

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