Bradykinin stimulates increased intracellular calcium in papillary collecting tubules of the rabbit

James A. Shayman; Keith A. Hruska; Aubrey R. Morrison

doi:10.1016/0006-291X(86)90562-0

Bradykinin stimulates increased intracellular calcium in papillary collecting tubules of the rabbit

James A. Shayman, Keith A. Hruska, Aubrey R. Morrison

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The effect of bradykinin on cytoplasmic Ca²⁺ concentration in rabbit papillary collecting tubule cells was determined using the fluorescent indicator Quin 2. Bradykinin stimulated a rapid increase in intracellular Ca²⁺. The rise in Ca²⁺ was dose dependent, persisted for less than 90 seconds and was independent of extracellular calcium. The ED₅₀ for bradykinin induced changes in [Ca²⁺]_i paralleled that observed previously for hormone-induced PGE₂ formation as well as for inositol trisphosphate labelling. These studies provide additional support for the role of Ca²⁺ as a second messenger for bradykinin in renal papillary collecting tubule cells.

Original language	English
Pages (from-to)	299-304
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	134
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(86)90562-0
State	Published - Jan 14 1986

Access to Document

10.1016/0006-291X(86)90562-0

Cite this

@article{9774d8f3a3854681a19514f44888108a,

title = "Bradykinin stimulates increased intracellular calcium in papillary collecting tubules of the rabbit",

abstract = "The effect of bradykinin on cytoplasmic Ca2+ concentration in rabbit papillary collecting tubule cells was determined using the fluorescent indicator Quin 2. Bradykinin stimulated a rapid increase in intracellular Ca2+. The rise in Ca2+ was dose dependent, persisted for less than 90 seconds and was independent of extracellular calcium. The ED50 for bradykinin induced changes in [Ca2+]i paralleled that observed previously for hormone-induced PGE2 formation as well as for inositol trisphosphate labelling. These studies provide additional support for the role of Ca2+ as a second messenger for bradykinin in renal papillary collecting tubule cells.",

author = "Shayman, {James A.} and Hruska, {Keith A.} and Morrison, {Aubrey R.}",

year = "1986",

month = jan,

day = "14",

doi = "10.1016/0006-291X(86)90562-0",

language = "English",

volume = "134",

pages = "299--304",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

number = "1",

}

TY - JOUR

T1 - Bradykinin stimulates increased intracellular calcium in papillary collecting tubules of the rabbit

AU - Shayman, James A.

AU - Hruska, Keith A.

AU - Morrison, Aubrey R.

PY - 1986/1/14

Y1 - 1986/1/14

N2 - The effect of bradykinin on cytoplasmic Ca2+ concentration in rabbit papillary collecting tubule cells was determined using the fluorescent indicator Quin 2. Bradykinin stimulated a rapid increase in intracellular Ca2+. The rise in Ca2+ was dose dependent, persisted for less than 90 seconds and was independent of extracellular calcium. The ED50 for bradykinin induced changes in [Ca2+]i paralleled that observed previously for hormone-induced PGE2 formation as well as for inositol trisphosphate labelling. These studies provide additional support for the role of Ca2+ as a second messenger for bradykinin in renal papillary collecting tubule cells.

AB - The effect of bradykinin on cytoplasmic Ca2+ concentration in rabbit papillary collecting tubule cells was determined using the fluorescent indicator Quin 2. Bradykinin stimulated a rapid increase in intracellular Ca2+. The rise in Ca2+ was dose dependent, persisted for less than 90 seconds and was independent of extracellular calcium. The ED50 for bradykinin induced changes in [Ca2+]i paralleled that observed previously for hormone-induced PGE2 formation as well as for inositol trisphosphate labelling. These studies provide additional support for the role of Ca2+ as a second messenger for bradykinin in renal papillary collecting tubule cells.

UR - http://www.scopus.com/inward/record.url?scp=0022650271&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(86)90562-0

DO - 10.1016/0006-291X(86)90562-0

M3 - Article

C2 - 3080988

AN - SCOPUS:0022650271

SN - 0006-291X

VL - 134

SP - 299

EP - 304

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Bradykinin stimulates increased intracellular calcium in papillary collecting tubules of the rabbit

Abstract

Access to Document

Other files and links

Fingerprint

Cite this