Bone cells: Biochemical and biological studies after enzymatic isolation

William A. Peck; Stanley J. Birge; Susan A. Fedak

doi:10.1126/science.146.3650.1476

Bone cells: Biochemical and biological studies after enzymatic isolation

William A. Peck
, Stanley J. Birge
, Susan A. Fedak

Division of Nutritional Science & Obesity Medicine

Research output: Contribution to journal › Article › peer-review

251 Scopus citations

Abstract

Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.

Original language	English
Pages (from-to)	1476-1477
Number of pages	2
Journal	Science
Volume	146
Issue number	3650
DOIs	https://doi.org/10.1126/science.146.3650.1476
State	Published - 1964

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title = "Bone cells: Biochemical and biological studies after enzymatic isolation",

abstract = "Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.",

author = "Peck, \{William A.\} and Birge, \{Stanley J.\} and Fedak, \{Susan A.\}",

year = "1964",

doi = "10.1126/science.146.3650.1476",

language = "English",

volume = "146",

pages = "1476--1477",

journal = "Science",

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number = "3650",

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TY - JOUR

T1 - Bone cells

T2 - Biochemical and biological studies after enzymatic isolation

AU - Peck, William A.

AU - Birge, Stanley J.

AU - Fedak, Susan A.

PY - 1964

Y1 - 1964

N2 - Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.

AB - Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.

UR - https://www.scopus.com/pages/publications/0001001573

U2 - 10.1126/science.146.3650.1476

DO - 10.1126/science.146.3650.1476

M3 - Article

C2 - 14208576

AN - SCOPUS:0001001573

SN - 0036-8075

VL - 146

SP - 1476

EP - 1477

JO - Science

JF - Science

IS - 3650

ER -

Bone cells: Biochemical and biological studies after enzymatic isolation

Abstract

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