Bone cells: Biochemical and biological studies after enzymatic isolation

William A. Peck; Stanley J. Birge; Susan A. Fedak

doi:10.1126/science.146.3650.1476

Bone cells: Biochemical and biological studies after enzymatic isolation

William A. Peck, Stanley J. Birge, Susan A. Fedak

Division of Nutritional Science & Obesity Medicine

Research output: Contribution to journal › Article › peer-review

244 Scopus citations

Abstract

Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.

Original language	English
Pages (from-to)	1476-1477
Number of pages	2
Journal	Science
Volume	146
Issue number	3650
DOIs	https://doi.org/10.1126/science.146.3650.1476
State	Published - 1964

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title = "Bone cells: Biochemical and biological studies after enzymatic isolation",

abstract = "Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.",

author = "Peck, {William A.} and Birge, {Stanley J.} and Fedak, {Susan A.}",

year = "1964",

doi = "10.1126/science.146.3650.1476",

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AU - Birge, Stanley J.

AU - Fedak, Susan A.

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AB - Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.

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M3 - Article

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JO - Science

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Bone cells: Biochemical and biological studies after enzymatic isolation

Abstract

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