TY - JOUR
T1 - Bogotá River anthropogenic contamination alters microbial communities and promotes spread of antibiotic resistance genes
AU - Posada-Perlaza, Carlos Eduardo
AU - Ramírez-Rojas, Adán
AU - Porras, Paola
AU - Adu-Oppong, Boahemaa
AU - Botero-Coy, Ana María
AU - Hernández, Félix
AU - Anzola, Juan M.
AU - Díaz, Lorena
AU - Dantas, Gautam
AU - Reyes, Alejandro
AU - Zambrano, María Mercedes
N1 - Funding Information:
Sampling was carried out under authorization by the Colombian National Authority of Environmental Licenses (ANLA) and participating hospitals. The authors thank Erica Pehrsson for providing the Lahey Clinic β-lactamase database and antibiotic metadata information; the sequencing service of the Center for Genome Sciences and Systems Biology in Washington University School of Medicine in St. Louis; and the High-Performance Computing Service at Universidad de los Andes in Bogotá, Colombia, for providing HPC resources that have contributed to the research results reported in this work (URL: http://hpc.uniandes.edu.co). Funding was provided by Colciencias (project No. 657065741359).
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The increase in antibiotic resistant bacteria has raised global concern regarding the future effectiveness of antibiotics. Human activities that influence microbial communities and environmental resistomes can generate additional risks to human health. In this work, we characterized aquatic microbial communities and their resistomes in samples collected at three sites along the Bogotá River and from wastewaters at three city hospitals, and investigated community profiles and antibiotic resistance genes (ARGs) as a function of anthropogenic contamination. The presence of antibiotics and other commonly used drugs increased in locations highly impacted by human activities, while the diverse microbial communities varied among sites and sampling times, separating upstream river samples from more contaminated hospital and river samples. Clinically relevant antibiotic resistant pathogens and ARGs were more abundant in contaminated water samples. Tracking of resistant determinants to upstream river waters and city sources suggested that human activities foster the spread of ARGs, some of which were co-localized with mobile genetic elements in assembled metagenomic contigs. Human contamination of this water ecosystem changed both community structure and environmental resistomes that can pose a risk to human health.
AB - The increase in antibiotic resistant bacteria has raised global concern regarding the future effectiveness of antibiotics. Human activities that influence microbial communities and environmental resistomes can generate additional risks to human health. In this work, we characterized aquatic microbial communities and their resistomes in samples collected at three sites along the Bogotá River and from wastewaters at three city hospitals, and investigated community profiles and antibiotic resistance genes (ARGs) as a function of anthropogenic contamination. The presence of antibiotics and other commonly used drugs increased in locations highly impacted by human activities, while the diverse microbial communities varied among sites and sampling times, separating upstream river samples from more contaminated hospital and river samples. Clinically relevant antibiotic resistant pathogens and ARGs were more abundant in contaminated water samples. Tracking of resistant determinants to upstream river waters and city sources suggested that human activities foster the spread of ARGs, some of which were co-localized with mobile genetic elements in assembled metagenomic contigs. Human contamination of this water ecosystem changed both community structure and environmental resistomes that can pose a risk to human health.
UR - http://www.scopus.com/inward/record.url?scp=85070866139&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-48200-6
DO - 10.1038/s41598-019-48200-6
M3 - Article
C2 - 31409850
AN - SCOPUS:85070866139
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 11764
ER -