Blood clearance rates of adenovirus type 5 in mice

R. Alemany, K. Suzuki, D. T. Curiel

Research output: Contribution to journalArticle

325 Scopus citations

Abstract

Persistence of adenovirus type 5 in blood has implications for the pathogenecity of the virus infection and for the use of this virus in oncolysis and gene therapy. In this study, the kinetics of adenovirus clearance from blood in mice has been evaluated. After a single inoculation of concentrated virus into the vena cava, virus half-life was less than 2 min. Depletion of Kupffer cells (KC) resulted in increased viraemia. After tail-vein injection, virus and latex beads co-localized within KC. An important factor in clearance by KC is the negative charge of particles. Deletion of the hexon hypervariable region 1 acidic stretch decreased the negative charge of the virion but it did not increase blood persistence. Coating with PEG ('PEGylation') reduced the clearance rate but also reduced infectivity.

Original languageEnglish
Pages (from-to)2605-2609
Number of pages5
JournalJournal of General Virology
Volume81
Issue number11
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

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