TY - JOUR
T1 - Bitter melon enhances natural killer-mediated toxicity against head and neck cancer cells
AU - Bhattacharya, Sourav
AU - Muhammad, Naoshad
AU - Steele, Robert
AU - Kornbluth, Jacki
AU - Ray, Ratna B.
N1 - Publisher Copyright:
© 2017 AACR.
PY - 2017/6
Y1 - 2017/6
N2 - Natural killer (NK) cells are one of the major components of innate immunity, with the ability tomediate antitumor activity. Understanding the role of NK-cell-mediated tumor killing in controlling of solid tumor growth is still in the developmental stage. We have shown recently that bitter melon extract (BME) modulates the regulatory T cell (Treg) population in head and neck squamous cell carcinoma (HNSCC). However, the role of BME in NK-cell modulation against HNSCC remains unknown. In this study, we investigated whether BME can enhance the NK-cell killing activity against HNSCC cells. Our results indicated that treatment of human NK-cell line (NK3.3) with BME enhances ability to kill HNSCC cells. BME increases granzyme B accumulation and translocation/accumulation of CD107a/ LAMP1 in NK3.3 cells exposed to BME. Furthermore, an increase in cell surface expression of CD16 and NKp30 in BME-treated NK3.3 cells was observed when cocultured with HNSCC cells. Collectively, our results demonstrated for the first time that BME augments NK-cell-mediated HNSCC killing activity, implicating an immunomodulatory role of BME.
AB - Natural killer (NK) cells are one of the major components of innate immunity, with the ability tomediate antitumor activity. Understanding the role of NK-cell-mediated tumor killing in controlling of solid tumor growth is still in the developmental stage. We have shown recently that bitter melon extract (BME) modulates the regulatory T cell (Treg) population in head and neck squamous cell carcinoma (HNSCC). However, the role of BME in NK-cell modulation against HNSCC remains unknown. In this study, we investigated whether BME can enhance the NK-cell killing activity against HNSCC cells. Our results indicated that treatment of human NK-cell line (NK3.3) with BME enhances ability to kill HNSCC cells. BME increases granzyme B accumulation and translocation/accumulation of CD107a/ LAMP1 in NK3.3 cells exposed to BME. Furthermore, an increase in cell surface expression of CD16 and NKp30 in BME-treated NK3.3 cells was observed when cocultured with HNSCC cells. Collectively, our results demonstrated for the first time that BME augments NK-cell-mediated HNSCC killing activity, implicating an immunomodulatory role of BME.
UR - http://www.scopus.com/inward/record.url?scp=85020264693&partnerID=8YFLogxK
U2 - 10.1158/1940-6207.CAPR-17-0046
DO - 10.1158/1940-6207.CAPR-17-0046
M3 - Article
C2 - 28465362
AN - SCOPUS:85020264693
SN - 1940-6207
VL - 10
SP - 337
EP - 343
JO - Cancer Prevention Research
JF - Cancer Prevention Research
IS - 6
ER -