TY - JOUR
T1 - Biosynthesis of terpene compounds using the non-model yeast Yarrowia lipolytica
T2 - grand challenges and a few perspectives
AU - Worland, Alyssa M.
AU - Czajka, Jeffrey J.
AU - Li, Yanran
AU - Wang, Yechun
AU - Tang, Yinjie J.
AU - Su, Wei Wen
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/8
Y1 - 2020/8
N2 - Yarrowia lipolytica has emerged as an important non-model host for terpene production. However, three main challenges remain in industrial production using this yeast. First, considerable knowledge gaps exist in metabolic flux across multiple compartments, cofactor generation, and catabolism of non-sugar carbon sources. Second, many enzymatic steps in the complex-terpene synthesis pathway can pose rate-limitations, causing accumulation of toxic intermediates and increased metabolic burdens. Third, metabolic shifts, morphological changes, and genetic mutations are poorly characterized under industrial fermentation conditions. To overcome these challenges, systems metabolic analysis, protein engineering, novel pathway engineering, model-guided strain design, and fermentation optimization have been attempted with some successes. Further developments that address these challenges are needed to advance the Yarrowia lipolytica platform for industrial-scale production of high-value terpenes, including those with highly complex structures such as anticancer molecules withanolides and insecticidal limonoids.
AB - Yarrowia lipolytica has emerged as an important non-model host for terpene production. However, three main challenges remain in industrial production using this yeast. First, considerable knowledge gaps exist in metabolic flux across multiple compartments, cofactor generation, and catabolism of non-sugar carbon sources. Second, many enzymatic steps in the complex-terpene synthesis pathway can pose rate-limitations, causing accumulation of toxic intermediates and increased metabolic burdens. Third, metabolic shifts, morphological changes, and genetic mutations are poorly characterized under industrial fermentation conditions. To overcome these challenges, systems metabolic analysis, protein engineering, novel pathway engineering, model-guided strain design, and fermentation optimization have been attempted with some successes. Further developments that address these challenges are needed to advance the Yarrowia lipolytica platform for industrial-scale production of high-value terpenes, including those with highly complex structures such as anticancer molecules withanolides and insecticidal limonoids.
UR - http://www.scopus.com/inward/record.url?scp=85083000829&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2020.02.020
DO - 10.1016/j.copbio.2020.02.020
M3 - Review article
C2 - 32299032
AN - SCOPUS:85083000829
SN - 0958-1669
VL - 64
SP - 134
EP - 140
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -